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苏州工业园区生物纳米园A4#216
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0512-67156496
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商品描述
Ubiquitin Linkage Screening Kit III is designed for two purposes: 1) to identify the ubiquitin lysine residue that is used to link the polyubiquitin chain conjugated on a protein substrate; 2) to determine whether a protein substrate is polyubiquitinated or conjugated with multiple monoubiquitin.N/A
Additional Information
| Product Name: | Ubiquitin Linkage Screening Kit III |
|---|---|
| Also Known As: | N/A |
| Catalog No.: | J3220 |
| Size: | 1 Kit |
| Molecular Weight: | N/A |
| Species: | Human |
| Source: | Bacterial recombinant |
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Concentration: | See tube label |
| Quality Assurance: | 95% based on SDS-PAGE |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF datasheet, MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | N/A |
| Shipping Method: | Dry ice shipping |
| References: | N/A |
Details
* The supplied UBE1 is sufficient for setting up 50 x 20 µl reactions. Each Ub mutant is sufficient for setting up 10 X 20 µl reactions.
Description
Ubiquitin Linkage Screening Kit III is designed for two purposes: 1) to identify the ubiquitin lysine residue that is used to link the polyubiquitin chain conjugated on a protein substrate; 2) to determine whether a protein substrate is polyubiquitinated or conjugated with multiple monoubiquitin.
A typical 20 µl reaction contains 100 nM UBE1, 2 µM E2 (not supplied), 50 µM Ub or Ub mutants, 4 mM ATP, 2 µM E3 (not supplied) and 2 µM substrate (no supplied) in the ubiquitination buffer.
Components
• 20X UBE1 (2 µM)..................................50 µl • 10X 6xHis-Ub (500 µM)..........................20 µl
• 10X 6xHis-Ub(K0) (500 µM)....................20 µl • 10X 6xHis-Ub(K6 only) (500 µM).............20 µl
• 10X 6xHis-Ub(K11 only) (500 µM)............20 µl • 10X 6xHis-Ub(K27 only) (500 µM)............20 µl
• 10X 6xHis-Ub(K29 only) (500 µM)............20 µl • 10X 6xHis-Ub(K33 only) (500 µM)............20 µl
• 10X 6xHis-Ub(K48 only) (500 µM)............20 µl • 10X 6xHis-Ub(K63 only) (500 µM)............20 µl
• 10X ATP (40 mM)....................................125 µl • 10X Ubiquitination Buffer...........................1 ml
Ubiquitin-Proteasome Biotechnologies成立于2011年，ubpbio是由科学家和投资者创立的私营企业。ubpbio致力于为学术界和工业界提供高质量的试剂和其他相关产品。ubpbio产品线包括的高度纯化的蛋白酶，E1和E2的酶，泛素和泛素衍生物，蛋白酶体抑制剂和蛋白酶基板。ubpbio不断开发创新以增加新的产品，ubpbio致力为全球的客户提供更多的帮助。在人类细胞中，有超过1000的蛋白质/酶，参与维持泛素-蛋白酶体系统的正常活动。失调的UPS已发现涉及大量的人类疾病包括癌症的病理生理，炎性疾病和神经退行性疾病。ubpbio致力为客户提供更好的泛素-蛋白酶体系统解决方案。 Description
Ubiquitin Linkage Screening Kit III is designed for two purposes: 1) to identify the ubiquitin lysine residue that is used to link the polyubiquitin chain conjugated on a protein substrate; 2) to determine whether a protein substrate is polyubiquitinated or conjugated with multiple monoubiquitin.
A typical 20 µl reaction contains 100 nM UBE1, 2 µM E2 (not supplied), 50 µM Ub or Ub mutants, 4 mM ATP, 2 µM E3 (not supplied) and 2 µM substrate (no supplied) in the ubiquitination buffer.
Components
• 20X UBE1 (2 µM)..................................50 µl • 10X 6xHis-Ub (500 µM)..........................20 µl
• 10X 6xHis-Ub(K0) (500 µM)....................20 µl • 10X 6xHis-Ub(K6 only) (500 µM).............20 µl
• 10X 6xHis-Ub(K11 only) (500 µM)............20 µl • 10X 6xHis-Ub(K27 only) (500 µM)............20 µl
• 10X 6xHis-Ub(K29 only) (500 µM)............20 µl • 10X 6xHis-Ub(K33 only) (500 µM)............20 µl
• 10X 6xHis-Ub(K48 only) (500 µM)............20 µl • 10X 6xHis-Ub(K63 only) (500 µM)............20 µl
• 10X ATP (40 mM)....................................125 µl • 10X Ubiquitination Buffer...........................1 ml
