Description

Cultrex®EmbryoidBodyFormationKitincludesROCKInhibitorY-27632,whichsignificantlyincreasessurvivalofPSCsinsinglecellculture,aspecializedEBFormationMatrixtostimulatecellaggregationanddevelopmentofEBs,anda96-wellEBFormationPlate,thatallowsforcontroloftheEBsizeandshape.Notransferstepisrequiredfordifferentiation.

Cultrex®EmbryoidFormationMatrixisapurifiedbasementmembraneproteinfromEngelbreth-Holm-Swarm(EHS)tumor.Cultrex®EmbryoidFormationMatrixhasbeendeveloped,producedandqualifiedtobeusedwithCultrex®EmbryoidFormationKit.

Features:

• HighEBgrowthsuccessrate
• UniformandconsistentsizedEBs
• Formdirectlyinplate,notransfersteprequired
• High-throughputformat
• ROCKInhibitorY-27632increasesPSCsurvivalrate
• SpecializedEBFormationMatrixstimulatescellaggregation

Qualitycontrolspecifications:

• Mousecoloniesareroutinelyscreenedforpathogensusing mouseantibodyproduction(MAP)testing.
• Engelbreth-Holm-Swarm(EHS)tumorisLDEV-free.
• Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
• Endotoxinconcentration≤20EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
• NomycoplasmacontaminationdetectedbyPCR.
• BIOLOGicalactivitydeterminedforeachlot:
  • EmbryoidBodyFormationAssay.

 

Catalog#3550-096-Kincludes:
CatalogNumber	Description	Qty
3550-096-01	Cultrex®10XEBFormationMatrix	1
3550-096-02	500XROCKinhibitorY-27632	1
3550-096-03	EBFormationPlate	1

Trevigen，Inc.是一家发展迅速的生物技术公司，致力于开发用于癌症研究，药物发现，遗传毒理学，再生医学和干细胞研究的产品和技术。我们提供试剂盒和试剂，用于研究DNA损伤和修复，细胞凋亡，氧化应激，癌细胞行为，3D培养和干细胞。该公司获得了美国国立卫生研究院（National Institutes of Health）多项SBIR资助。