Succinimidyl-2-(biotinamido)-ethyl-1,3"-dithiopropionate(NHS-SS-Biotin) is an amine-reactive biotinylation reagent that is cleavable when the disulfide (-S-S-) bond is reduced.NHS-SS-Biotin is cell membrane permeable and has a long chain separating the biotin group from the amino-reactive NHS-ester (N-Hydroxysuccinimide ester).It is possible to cleave the biotin group from the crosslinked target protein under reducing conditions, such as in the presence of beta-mercaptoethanol (BME), DTT or TCEP. This technique is often used to purify an antibody or other protein from a biotin specific affinity resin like Immobilized Streptavidin without exposing your protein of interest to denaturing conditions.

Click here for: NHS-SS-Biotin Protocol& Product Information Sheet

Molecular Weight: 504.65Spacer Length: 24.3 ÅCAS Number: 142439-92-7Alternate Names: Biotin disulfide N-Hydroxysuccinimide ester; NHS SS BiotinSpecial bulk pricing is available on bulk quantities of NHS-SS-Biotin for your large volume manufacturing application.

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