ProteinTech/RSK2多克隆抗体/150ul/23762-1-AP

价格
¥1980.00
货号:23762-1-AP
浏览量:127
品牌:ProteinTech
服务
全国联保
正品保证
正规发票
签订合同
商品描述

Tested Applications

Positive WB detected inNIH/3T3 cells, K-562 cells,MCF-7 cells,HepG2 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman kidney tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

The immunogen of 23762-1-AP is RSK2 Fusion Protein expressed in E. coli.

Tested Reactivityhuman,mouse
Cited Reactivity human
Host / IsotypeRabbit / IgG
ClassPolyclonal
TypeAntibody
ImmunogenRSK2 fusion protein Ag20620
Full Nameribosomal protein S6 kinase, 90kDa, polypeptide 3
Calculated molecular weight 740 aa, 84 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC096301
Gene symbolRPS6KA3
Gene ID (NCBI)6197
ConjugateUnconjugated
FormLiquid
Purification MethodAntigen affinity purification
Storage BufferPBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.

Background Information

RPS6KA3(Ribosomal protein S6 kinase alpha-3) is also named as ISPK1, MAPKAPK1B, RSK2 and belongs to the protein kinase superfamily. It localizes at the kinetochores under spindle checkpoint conditions, and during mitosis, associated with the centrosomes, the spindle and at the mid-body(PMID:20383198). It is required for EGF activated phosphorylation of histone H3, which may cause chromatin remodeling and facilitate gene transcription and maybe playing an essential regulatory role in protein synthesis during cell proliferation.

Protocols

Product Specific Protocols
WB protocol for RSK2 antibody 23762-1-APDownload protocol
IHC protocol for RSK2 antibody 23762-1-APDownload protocol
IF protocol for RSK2 antibody 23762-1-APDownload protocol
IP protocol for RSK2 antibody 23762-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
Proteintech 抗体的更高识别能力并没有比其抗 SMN2 抗体 (11708-1-AP) 更好地证明。SMA(脊髓性肌萎缩症)基金会的研究人员着手开发一种仅需要一毫升血液即可检测 SMN2 的分析方法。由于 SMA 患者的 SMN2 水平非常低,因此很难判断。其中存在一个挑战,因为蛋白质信号不能像通过 PCR 检测 DNA 的痕迹一样从小样本中增强。然而,使用具有高亲和力的敏感抗体,可以捕获和富集蛋白质以进行检测。这是夹心 ELISA 程序的基本原理,全蛋白产生的抗体是此类协议的理想候选者。这是因为它们以三维的天然构象识别出更多的蛋白质。在他们努力开发用于检测 PBMC 中 SMN2 的夹心 ELISA 测定法时,SMA 基金会的科学家们发现,Proteintech 的抗 SMN2 抗体 (11708-1-AP) 的灵敏度是其竞争对手中最接近的同类抗体的四倍1。将这种抗体推进到他们的检测设计中,SMA 研究人员能够开发出一种可靠、定量和快速的方法来测量 SMA 患者 PBMC 中的 SMN2。此外,由此产生的测定还能够检测小鼠来源的几种组织中的 SMN,包括严重 SMA 的小鼠模型。