ProteinTech/CAMP多克隆抗体/150ul/12009-1-AP

价格
¥1980.00
货号:12009-1-AP
浏览量:127
品牌:ProteinTech
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商品描述

Tested Applications

Positive IHC detected inhuman tonsillitis tissue,human spleen tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KOSee 1 publications below
WBSee 2 publications below
IHCSee 2 publications below

Product Information

12009-1-AP targets CAMP inWB, IHC, IF, ELISA applications and shows reactivity with human,mouse samples.

Tested Reactivityhuman,mouse
Cited Reactivity mouse, rat
Host / IsotypeRabbit / IgG
ClassPolyclonal
TypeAntibody
ImmunogenCAMP fusion protein Ag2622
Full Namecathelicidin antimicrobial peptide
Calculated molecular weight 170 aa, 19 kDa
GenBank accession numberBC055089
Gene symbolCAMP
Gene ID (NCBI)820
ConjugateUnconjugated
FormLiquid
Purification MethodAntigen affinity purification
Storage BufferPBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.

Background Information

CAMP is a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. CAMP encodes the 18-kDa proprotein hCAP18. FALL-39 and LL-37 are the mature cathelicidin peptides. This antibody can recognize all the proprotein and cleaved species.

Protocols

Product Specific Protocols
IHC protocol for CAMP antibody 12009-1-APDownload protocol
IF protocol for CAMP antibody 12009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
Proteintech 抗体的更高识别能力并没有比其抗 SMN2 抗体 (11708-1-AP) 更好地证明。SMA(脊髓性肌萎缩症)基金会的研究人员着手开发一种仅需要一毫升血液即可检测 SMN2 的分析方法。由于 SMA 患者的 SMN2 水平非常低,因此很难判断。其中存在一个挑战,因为蛋白质信号不能像通过 PCR 检测 DNA 的痕迹一样从小样本中增强。然而,使用具有高亲和力的敏感抗体,可以捕获和富集蛋白质以进行检测。这是夹心 ELISA 程序的基本原理,全蛋白产生的抗体是此类协议的理想候选者。这是因为它们以三维的天然构象识别出更多的蛋白质。在他们努力开发用于检测 PBMC 中 SMN2 的夹心 ELISA 测定法时,SMA 基金会的科学家们发现,Proteintech 的抗 SMN2 抗体 (11708-1-AP) 的灵敏度是其竞争对手中最接近的同类抗体的四倍1。将这种抗体推进到他们的检测设计中,SMA 研究人员能够开发出一种可靠、定量和快速的方法来测量 SMA 患者 PBMC 中的 SMN2。此外,由此产生的测定还能够检测小鼠来源的几种组织中的 SMN,包括严重 SMA 的小鼠模型。