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Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells,MDCK cells,NIH/3T3 cells |
| Positive IP detected in | knockout cells and WT cells,HEK-293 cells |
| Positive IHC detected in | human heart tissue, human pancreas tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MDCK cells, hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 27 publications below |
| WB | See 84 publications below |
| IHC | See 1 publications below |
| IF | See 159 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
13967-1-AP targets IFT88 inWB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat,dog samples.
| Tested Reactivity | human, mouse, rat,dog |
| Cited Reactivity | Chick, Chicken, dog, human, mouse, rat, swine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IFT88 fusion protein Ag4980 |
| Full Name | intraflagellar transport 88 homolog (Chlamydomonas) |
| Calculated molecular weight | 94 kDa |
| Observed molecular weight | 88-95 kDa |
| GenBank accession number | BC030776 |
| Gene symbol | IFT88 |
| Gene ID (NCBI) | 8100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFT88 antibody 13967-1-AP | Download protocol |
| IHC protocol for IFT88 antibody 13967-1-AP | Download protocol |
| IF protocol for IFT88 antibody 13967-1-AP | Download protocol |
| IP protocol for IFT88 antibody 13967-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
