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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Specifications
| TESTEDAPPLICATIONS: | ELISA |
|---|---|
| SETCONTENTS: | Thissetincludeacapturemonoclonalantibody[7F4],abiotin-labeleddetectionmonoclonalantibody[8G9],andarecombinantHIV-1p24proteinstandard. |
| APPLICATIONS: | ThissetcanbeusedtomonitortherateofHIV-1productioninvitroorforthedetectionofHIV-1.Itcandetectp24fromdifferentHIV-1subtypeswithcomparablesensitivity.ThedetectionsethasbeenoptimizedandvalidatedforHIVresearch. CaptureAntibody:HIV-1p24Antibody[7F4],Cat.No.PM-6585 Thisantibodywasraisedagainstarecombinantfull-lengthHIV-1p24protein.Thesuggestedcoatingconcentrationinap24sandwichELISAis5-10µg/ml. DetectionAntibody:HIV-1p24Antibody[8G9],Cat.No.PM-6335-biotin Thisantibodywasraisedagainstarecombinantfull-lengthHIV-1p24proteinandconjugatedwithbiotin.Thesuggestedconcentrationinasandwichp24ELISAis0.125-0.25µg/ml. ProteinStandard:HIV-1p24RecombinantProtein,Cat.No.95-153 Thisproteinistherecombinantfull-lengthHIV-1p24fromHxB2strain(aa133-364)expressedandpurifiedfromSaccharomycescerevisiae.Therecombinantproteinisusedasastandardforcalibrationofthep24ELISA. |
| USERNOTE: | Setisavailablein3differentsizeswithenoughreagentsfor1,5,or1096-wellplates. |
SetContents
Properties
| PHYSICALSTATE: | Liquid |
|---|---|
| STORAGECONDITIONS: | StoreHIV-1p24MonoclonalAntibodiesat-20˚C.StoreHIV-1p24recombinantproteinat-70˚C.Avoidrepeatedfreeze/thawcycles. |
AdditionalInfo
Background
| Thehumanimmunodeficiencyvirustype1(HIV-1)particleconsistsofanenvelope,acoreandtheregionbetweenthetwotermedmatrix(1).TheHIV-1Gagproteinisalatestructuralproteinthatcontainsfourproteins:matrix(p17),capsid(p24),nucleocapsid(p7)andthep6protein(2).Thep24constitutesthemajorcorecomponentofthevirusandshowshighdegreeofsequenceconservationamongHIVisolates.TheGagp24hasbeenusedasanintegralpartofmulticomponentHIV-1vaccines(3). References: 1)PMID:9684349 GotoT,NakaiM,andIkutaK.Thelife-cycleofhumanimmunodeficiencyvirustype1.Micron1998;29:123-38. 2)PMID:9813197 FreedEO.HIV-1gagproteins:diversefunctionsintheviruslifecycle.Virology1998;251:1-15. 3)PMID:21467219 FlynnBJ,KastenmullerK,Wille-ReeceU,etal.ImmunizationwithHIVGagtargetedtodendriticcellsfollowedbyrecombinantNewYorkvacciniavirusinducesrobustT-cellimmunityinnonhumanprimates.PNAS2011;108(17):7131–6. |
