PromoCell Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells.

Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for horse and porcine keratinocytes, as well as mouse and rat keratinocytes after adapting the CaCl2 concentration. In addition, it is suited for rat corneal keratinocytes.

To view a list of references where this medium was used with other species/cell types please click here.

Components:

• Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix
• Growth Medium Kit: Includes Basal Medium and SupplementPack
• SupplementMix: Contains all media supplements premixed in one vial
• SupplementPack: Contains all media supplements as individual vials

Media supplement	Final supplement concentration
Bovine Pituitary Extract	0.004 ml / ml
Epidermal Growth Factor (recombinant human)	0.125 ng / ml
Insulin (recombinant human)	5 μg / ml
Hydrocortisone	0.33 μg / ml
Epinephrine	0.39 μg / ml
Transferrin (recombinant human)	10 μg / ml
CaCl2	0.06 mM

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