- Extract total RNA, including virus & viroid RNA
- Robust lysis buffer is well-suited to even challenging samples such as pine needle, grape leaf, etc
- Isolate total RNA (including microRNA) without phenol
- Isolated RNA is of high quality, integrity and diversity
- Also available in 96-well format for high throughput applications
Figure 1. Isolation of High Quality RNA, even from Difficult Samples. Total RNA was isolated from 50 mg samples of apple (Lanes 1), peach (Lanes 2), grape (Lanes 3), pine needle (Lanes 4), strawberry (Lanes 5) and pear (Lanes 6) using Norgen"s kit and a competitors kit. Five microlitres of total RNA from the 50 µL elution was loaded on 1X MOPS 1.0 % Formaldehyde-Agarose RNA gel for analysis. Norgen"s kit allowed for the isolation of high quality RNA from all the samples, including the difficult samples, while the competitor failed to isolate RNA from grape, pine needles and strawberry. Furthermore, only Norgen"s kit was able to isolate the small RNA species (white box).]">
Plant/Fungi Total RNA Purification Kit Figure 1
Figure 2. Detection of EF1-α using one step RT-qPCR from Challenging Plant Samples. Total RNA was isolated from 50 mg samples of apple (red), peach (green), pine needle (blue) and grape leaves (burgundy) using Norgen"s Plant/Fungi Total RNA Purification Kit. Three µL of the eluted 50 µL of RNA was used in an RT-qPCR reaction for the detection of EF1-α. EF1-α was detected from all samples, indicating that the RNA is of high quality and that the Plant/Fungi RNA Purification kit is highly sensitive for total RNA isolation.
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Plant/Fungi Total RNA Purification Kit Figure 2
Figure 3. High Yield of Plant RNA Samples isolated from Apple, Peach and Pear. Total RNA was isolated using Norgen"s kit (Blue bars) and acompetitors kit (Dark blue bars) from 50 mg of apple, peach and pear according to the provided manual. In each case the RNA was eluted into 50 µL. RNA yield was measured by spectrophotometer in triplicate, and the average concentration was used for data analysis. Norgen"s kit resulted in higher yields of total RNA, including miRNA. Total processing time was less with Norgen"s kit than competitor"s kit.]">
Plant/Fungi Total RNA Purification Kit Figure 3
Figure 4. High Purity of RNA Samples Isolated from Apple, Peach and Pear. RNA was isolated from 50 mg samples of apple, peach and pear using Norgen"s Plant/Fungi RNA Purification Kit and a competitor"s kit. RNA purity was determined spectrophotometrically for the RNA isolated using Norgen"s kit (260/230 = dark blue bars) and the competitor"s kit (260/230 = light blue bars). Also shown is the 260/280 ratio for the samples. Norgen"s kit consistently isolated pure samples of Plant RNA with 260/230 ratios above 2.0, whereas the competitor"s kit had 260/230 ratios below 1.2 for the same samples.]">
Plant/Fungi Total RNA Purification Kit Figure 4
Plant/Fungi Total RNA Purification Kit
Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient.
The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen"s Plant/Fungi Total RNA Purification Kit is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
加拿大 . Norgen Biotek
Norgen Biotek
加拿大Norgen Biotek Corp www.norgenbiotek.com
Norgen Biotek是来自加拿大的著名生物技术公司，生产基于专利技术上的核酸和蛋白质纯化及富集产品。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖。目前该公司的产品多达40余种，覆盖基因组和蛋白组，广泛地被用于药厂及科研院所研究和新产品开发的各个方面。
Mission statement:
To provide an innovative range of first class RNA, DNA and p rotein purification, clean-up and concentration kits; and to provide dedicated and expert support services to our cus tomers and commercial partners worldwide.
Quality Policy:
The team at Norgen Biotek Corp. is committed to providing our customers with first class sample preparation kits for RNA, DNA and protein purification, clean-up and concentration for use in research applications, and to provide dedicated and expert support services to our cus tomers and partners worldwide.
Norgen Biotek Corp will achieve this through our commitment to:
Providing high quality, first-class, cost effective products;
Continuous improvements to our quality system, our manufacturing operations, our business practices and our products;
Continuous improvements to cus tomer satisfaction by maintaining a customer feedback process to capture and analyze customer needs and feedback on product effectiveness;
Identification, resolution, correction and prevention of quality problems
Educating and training employees to accomplish quality goals
Maintaining our Quality Management System so as to conform to ISO 13485:2003, and comply with all relevant regulatory requirements.
Values:
The values that drive the activities of Norgen Biotek are:
A commitment at all levels to the continuous improvement of our Quality Management System.
A commitment to teamwork. We will benefit from the increased potential of employees working together to solve problems and generate new ideas.
A commitment to creating a positive work atmosphere, where individuals are encouraged to develop professionally and personally through continuous learning, and where performance is recognized and rewarded.
We are committed to always conduct our business and manufacturing practices in an open and ethical manner.
We are committed to always treat our cus tomers, our coworkers and our suppliers with respect.
Company Overview:
Norgen Biotek Corp. is an innovative, biotechnology company focusing principally on advancing novel, innovative sample preparation products for nucleic acid (DNA & RNA) and protein purification, clean-up and concentration. Norgen has one platform technology with many applications based on a proprietary resin that can be applied in the purification of DNA, RNA and proteins. The uniqueness and novelty of this technology has been recognized by the National Research Council through awarding Norgen the prestigious “Innovation Leader Award for 2003”. The company holds more than a dozen issued patents and several others are pending. Norgen currently manufactures over 40 products that are used by researchers working in genomics and proteomics in academia, pharma/biotech companies, and government institutions. These products are based on Norgen’s novel technology and include:
1. RNA purification kits : Norgen manufactures the leading products in the world for the purification of total RNA, microRNA, cy toplasmic and nuclear RNA, and RNA from the exfoliated cells found in urine. Norgen’s RNA kits are the only kits in the world that isolate true total RNA (including microRNA) without the use of phenol extraction steps. Simply stated, our kits are currently the Gold Standard in the RNA purification area, offering many significant competitive advantages. Due to the fact that Norgen’s RNA purification products do not involve phase separation based on the use of phenol, they can easily be adapted to isolate total RNA, including microRNA, in high throughput. In addition, Norgen is also pioneering kits for macromolecule fractionation using one column without sample splitting. Towards this goal, Norgen has developed the RNA/DNA/Protein Kit (3-in-1) for purification of all 3 macromolecules from one sample sequentially. In addition, a recent product has been developed that added microRNA fractionation to the RNA/DNA/Protein Kit, termed the All-in-One Purification Kit (4-in-1). These products are unique and novel and offer a substantial cost and time savings over sample splitting and a several-column approach. These powerful and novel tools are designed for researchers who are interested in studying the genome, proteome and transcriptome of a single sample, such as for studies of gene expression including gene silencing experiments or mRNA knock-downs and studies involving biomarker discovery.
2. ProteoSpin™ brand protein kits : For desalting, detergent and endotoxin removal, concentration, inclusion body screening, abundant serum protein depletion, urine protein concentration and on-column peptide preparation for mass spec analysis.
3. DNA kits: For plasmid isolation, PCR purification, gel extraction, eukaryotic and prokaryotic genomic DNA isolation, and front-end sample preparation for diagnostics and forensic applications (including milk bacterial DNA isolation, urine bacterial DNA isolation, saliva DNA isolation, and urine DNA isolation).
4. Molecular weight ladders : A broad range of DNA, RNA and protein ladders are available.
Company Location
Norgen is located in a new, 24,000 square foot state-of-the-art facility in the middle of Niagara region, Ontario, Canada one block from Brock University. We are only 30 minutes from Buffalo and 1 hour from Toron to. Also, Niagara Falls and the wineries of Niagara-on-the-Lake are located within 10 minutes of the facility. The building itself is on a large property adjacent to the 406 Highway in Thorold’s technology district, and the property offers much room for expansion.
