Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format)
Norgen's Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 2 mL. Norgen's Plasma/Serum Circulating and Exosomal RNA Purification 96-Well Kit (Slurry Format) provides a high throughput method for isolating circulating and exosomal RNA from plasma and serum samples using either a vacuum manifold or centrifugation. These kits are able to isolate all sizes of circulating RNA and exosomal RNA, including microRNA, without the use of phenol or chloroform. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating and exosomal RNA from large sample volumes. RNA can be isolated from either fresh or frozen samples using these kits. These kits are suitable for the isolation of RNA from serum or plasma prepared from blood collected only on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications including RT-PCR.
The purified plasma/serum free-circulating and exosomal RNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
Free-circulating and exosomal plasma and serum RNA can serve as both tumor- and fetal-specific markers for cancer detection and prenatal diagnosis. As well, free-circulating and exosomal RNAs have the potential to provide biomarkers for other disease states. Free-circulating and exosomal RNA in plasma or serum are usually present as short fragments of less than 1000nt, and free-circulating miRNA (21nt) can also be found in plasma and serum.
Figure 1. Isolation and Detection of Circulating RNA from Different Plasma Volumes. Norgen's Plasma/Serum Circulating RNA Purification Mini Kit (Slurry Format) was used to isolate circulating RNA from 0.5mL, 1mL and 2mL plasma. Three microlitres of the purified RNA was then used as the template in RT-qPCR reactions to detect the human 5S gene. The 5S housekeeping gene was detected from all plasma sample volumes used. The amplification of the 5S rRNA showed an increasing amount of RNA with increasing the sample input volume. This is represented by the decrease of the Ct value with increasing the sample input volume. RNA isolated from 0.5mL plasma is represented by the Red line, RNA isolated from 1mL plasma is represented by the Green line whereas RNA isolated from 2mL plasma is represented by the Blue line. The black line corresponds to the no template control.
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Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) Figure 1
Figure 2. Effective Isolation of Plasma Circulating RNA from Different Volumes. Norgen's Plasma/Serum Circulating RNA Purification Mini Kit (Slurry Format) was used to isolate circulating RNA from 0.5mL, 1mL and 2mL plasma. Three microlitres of the purified RNA was then used as the template in RT-qPCR reactions to detect the human 5S gene. The 5S housekeeping gene was detected from all plasma sample volumes used. The amplification of the 5S rRNA showed an increasing amount of RNA with increasing the sample input volume. This is represented by the decrease of the Ct value with increasing the sample input volume. Average Ct. values for the amplification of the 5S rRNA isolated from 0.5mL plasma is represented by the Red bar, Average Ct. values for the amplification of the 5S rRNA isolated from 1mL plasma is represented by the Green bar whereas Average Ct. values for the amplification of the 5S rRNA isolated from 2mL plasma is represented by the blue bar.]">

Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) Figure 2
Figure 3.Effective and Consistent Detection of Plasma Exosome RNA.Norgen'sPlasma/Serum Circulating and Exosomal RNA Isolation Kits can effectively isolate RNA from plasma. Plasma Exosome RNA was isolated from 500 µL of human plasma prepared from blood collected on citrate, EDTA or Heparin in triplicates using Norgen's Plasma/Serum RNA Isolation Kit (blue), a ExoQuick Exosome Precipitation Reagent (green) and Qiagens QIAzol extraction followed by a modified Qiagen's RNeasy Mini Kit cleanup (red). Stem loop RT-qPCR using primers specific to miR-21 and miR-16 as well as the housekeeping 5S rRNA was performed. In brief, three microliters of the 100 µL isolated RNA was then subjected to a 20 µL reverse transcription using 5S rRNA, miR-21 and miR-16 stem-loop reverse primer or reveres primer. Three microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21, human miR-16 and the 5S rRNA. Norgen's Plasma/Serum Exosome RNA Isolation Kit is the only product that showed consistent detection of all tested transcripts with the highest quality regardless the type of the anti-coagulant used for blood collection.]">

Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) Figure 3
加拿大 . Norgen Biotek
Norgen Biotek
加拿大Norgen Biotek Corp www.norgenbiotek.com
Norgen Biotek是来自加拿大的著名生物技术公司,生产基于专利技术上的核酸和蛋白质纯化及富集产品。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖。目前该公司的产品多达40余种,覆盖基因组和蛋白组,广泛地被用于药厂及科研院所研究和新产品开发的各个方面。
Mission statement:
To provide an innovative range of first class RNA, DNA and p rotein purification, clean-up and concentration kits; and to provide dedicated and expert support services to our cus tomers and commercial partners worldwide.
Quality Policy:
The team at Norgen Biotek Corp. is committed to providing our customers with first class sample preparation kits for RNA, DNA and protein purification, clean-up and concentration for use in research applications, and to provide dedicated and expert support services to our cus tomers and partners worldwide.
Norgen Biotek Corp will achieve this through our commitment to:
Providing high quality, first-class, cost effective products;
Continuous improvements to our quality system, our manufacturing operations, our business practices and our products;
Continuous improvements to cus tomer satisfaction by maintaining a customer feedback process to capture and analyze customer needs and feedback on product effectiveness;
Identification, resolution, correction and prevention of quality problems
Educating and training employees to accomplish quality goals
Maintaining our Quality Management System so as to conform to ISO 13485:2003, and comply with all relevant regulatory requirements.
Values:
The values that drive the activities of Norgen Biotek are:
A commitment at all levels to the continuous improvement of our Quality Management System.
A commitment to teamwork. We will benefit from the increased potential of employees working together to solve problems and generate new ideas.
A commitment to creating a positive work atmosphere, where individuals are encouraged to develop professionally and personally through continuous learning, and where performance is recognized and rewarded.
We are committed to always conduct our business and manufacturing practices in an open and ethical manner.
We are committed to always treat our cus tomers, our coworkers and our suppliers with respect.
Company Overview:
Norgen Biotek Corp. is an innovative, biotechnology company focusing principally on advancing novel, innovative sample preparation products for nucleic acid (DNA & RNA) and protein purification, clean-up and concentration. Norgen has one platform technology with many applications based on a proprietary resin that can be applied in the purification of DNA, RNA and proteins. The uniqueness and novelty of this technology has been recognized by the National Research Council through awarding Norgen the prestigious “Innovation Leader Award for 2003”. The company holds more than a dozen issued patents and several others are pending. Norgen currently manufactures over 40 products that are used by researchers working in genomics and proteomics in academia, pharma/biotech companies, and government institutions. These products are based on Norgen’s novel technology and include:
1. RNA purification kits : Norgen manufactures the leading products in the world for the purification of total RNA, microRNA, cy toplasmic and nuclear RNA, and RNA from the exfoliated cells found in urine. Norgen’s RNA kits are the only kits in the world that isolate true total RNA (including microRNA) without the use of phenol extraction steps. Simply stated, our kits are currently the Gold Standard in the RNA purification area, offering many significant competitive advantages. Due to the fact that Norgen’s RNA purification products do not involve phase separation based on the use of phenol, they can easily be adapted to isolate total RNA, including microRNA, in high throughput. In addition, Norgen is also pioneering kits for macromolecule fractionation using one column without sample splitting. Towards this goal, Norgen has developed the RNA/DNA/Protein Kit (3-in-1) for purification of all 3 macromolecules from one sample sequentially. In addition, a recent product has been developed that added microRNA fractionation to the RNA/DNA/Protein Kit, termed the All-in-One Purification Kit (4-in-1). These products are unique and novel and offer a substantial cost and time savings over sample splitting and a several-column approach. These powerful and novel tools are designed for researchers who are interested in studying the genome, proteome and transcriptome of a single sample, such as for studies of gene expression including gene silencing experiments or mRNA knock-downs and studies involving biomarker discovery.
2. ProteoSpin™ brand protein kits : For desalting, detergent and endotoxin removal, concentration, inclusion body screening, abundant serum protein depletion, urine protein concentration and on-column peptide preparation for mass spec analysis.
3. DNA kits: For plasmid isolation, PCR purification, gel extraction, eukaryotic and prokaryotic genomic DNA isolation, and front-end sample preparation for diagnostics and forensic applications (including milk bacterial DNA isolation, urine bacterial DNA isolation, saliva DNA isolation, and urine DNA isolation).
4. Molecular weight ladders : A broad range of DNA, RNA and protein ladders are available.
Company Location
Norgen is located in a new, 24,000 square foot state-of-the-art facility in the middle of Niagara region, Ontario, Canada one block from Brock University. We are only 30 minutes from Buffalo and 1 hour from Toron to. Also, Niagara Falls and the wineries of Niagara-on-the-Lake are located within 10 minutes of the facility. The building itself is on a large property adjacent to the 406 Highway in Thorold’s technology district, and the property offers much room for expansion.