- Cleans and concentrates single-stranded or double-stranded DNA or RNA oligonucleotides larger than 10 bases
- Rapid and efficient spin column procedure
- No phenol, chloroform or alcohol precipitations are involved
- High recovery of up to 90%
- Efficient removal of enzymatic reaction buffers and proteins
- Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
Figure 1. Integrity of Purified DNA Oligonucleotides. Oligonucleotides of different sizes (15mer, 20mer, 42mer and 61mer) were purified using Norgen"s Oligo Clean-Up and Concentration Kit, and the integrity of the oligonucleotides before and after cleaning were compared by running a 15% urea-PAGE gel. Equal volumes of the input (I) and cleaned (C) oligonucleotide were run, and as it can be seen the purified oligonucleotides were of a high quality and integrity. Please note that the kit can also clean a mixture of different oligo sizes, as shown on the far right two lanes.]">

Figure 1.High Integrity of Purified DNA Oligonucleotides
Figure 2. Integrity of Purified dsDNA. Samples of Norgen"s MiniSizer DNA ladder (25bp-650bp) were purified using Norgen"s Oligo Clean-Up and Concentration Kit, and the integrity of the dsDNA before and after cleaning was compared by running aliquots on a 2% agarose gel. Both the input volume and the elution volume were maintained at 50 µL, and 5 µL and 10 µL of the input and 5 µL and 10 µL of the elution were run on the gel for visual comparison. As it can be seen, the purified dsDNA was of a high quality and integrity. The overall recovery of the different ladder bands is 82% of the input.
]">

Figure 2.Integrity of Purified dsDNA
Figure 3. Integrity of Purified RNA. Samples of Norgen"s 100b RNA ladder (100b-1000b) were purified using Norgen"s Oligo Clean-Up and Concentration Kit, and the integrity of the RNA before and after cleaning was compared by running aliquots of the input and elution on a 1.4% formaldehyde-agarose gel. Both the input volume and elution were maintained at 50 µL, and 5 µL and 10 µL of the input and 5 µL and 10 µL of the elution were run on the gel for visual comparison. The kit provides efficient RNA recovery of all the ladder bands. The overall recovery of the different ladder bands is 84% of the input.]">

Figure 3.High Integrity of Purified RNA
Oligo Clean-Up and Concentration Kit
This kit provides a rapid spin column procedure for the purification and concentration of up to 10 µg of oligonucleotides of various sizes. The kit is used for the purification of synthesized oligos, as well as the clean-up of oligos from various upstream enzymatic reactions such as ligation, Poly(A) tailing and end-labeling.
Single or double stranded DNA or RNA oligos larger than 10 bp can be purified with this kit (not recommended for the removal of PCR primers). Oligos of up to 1000 bp have been tested with this kit.
This kit purifies oligos from other reaction components including proteins, buffers and nucleotides without the use of phenol, chloroform, alcohol precipitation or urea PAGE extraction.
The kit provides a high quality product with up to 90% recovery. The purified oligos can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, in situ hybridization, and RNAi studies.
加拿大 . Norgen Biotek
Norgen Biotek
加拿大Norgen Biotek Corp www.norgenbiotek.com
Norgen Biotek是来自加拿大的著名生物技术公司,生产基于专利技术上的核酸和蛋白质纯化及富集产品。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖。目前该公司的产品多达40余种,覆盖基因组和蛋白组,广泛地被用于药厂及科研院所研究和新产品开发的各个方面。
Mission statement:
To provide an innovative range of first class RNA, DNA and p rotein purification, clean-up and concentration kits; and to provide dedicated and expert support services to our cus tomers and commercial partners worldwide.
Quality Policy:
The team at Norgen Biotek Corp. is committed to providing our customers with first class sample preparation kits for RNA, DNA and protein purification, clean-up and concentration for use in research applications, and to provide dedicated and expert support services to our cus tomers and partners worldwide.
Norgen Biotek Corp will achieve this through our commitment to:
Providing high quality, first-class, cost effective products;
Continuous improvements to our quality system, our manufacturing operations, our business practices and our products;
Continuous improvements to cus tomer satisfaction by maintaining a customer feedback process to capture and analyze customer needs and feedback on product effectiveness;
Identification, resolution, correction and prevention of quality problems
Educating and training employees to accomplish quality goals
Maintaining our Quality Management System so as to conform to ISO 13485:2003, and comply with all relevant regulatory requirements.
Values:
The values that drive the activities of Norgen Biotek are:
A commitment at all levels to the continuous improvement of our Quality Management System.
A commitment to teamwork. We will benefit from the increased potential of employees working together to solve problems and generate new ideas.
A commitment to creating a positive work atmosphere, where individuals are encouraged to develop professionally and personally through continuous learning, and where performance is recognized and rewarded.
We are committed to always conduct our business and manufacturing practices in an open and ethical manner.
We are committed to always treat our cus tomers, our coworkers and our suppliers with respect.
Company Overview:
Norgen Biotek Corp. is an innovative, biotechnology company focusing principally on advancing novel, innovative sample preparation products for nucleic acid (DNA & RNA) and protein purification, clean-up and concentration. Norgen has one platform technology with many applications based on a proprietary resin that can be applied in the purification of DNA, RNA and proteins. The uniqueness and novelty of this technology has been recognized by the National Research Council through awarding Norgen the prestigious “Innovation Leader Award for 2003”. The company holds more than a dozen issued patents and several others are pending. Norgen currently manufactures over 40 products that are used by researchers working in genomics and proteomics in academia, pharma/biotech companies, and government institutions. These products are based on Norgen’s novel technology and include:
1. RNA purification kits : Norgen manufactures the leading products in the world for the purification of total RNA, microRNA, cy toplasmic and nuclear RNA, and RNA from the exfoliated cells found in urine. Norgen’s RNA kits are the only kits in the world that isolate true total RNA (including microRNA) without the use of phenol extraction steps. Simply stated, our kits are currently the Gold Standard in the RNA purification area, offering many significant competitive advantages. Due to the fact that Norgen’s RNA purification products do not involve phase separation based on the use of phenol, they can easily be adapted to isolate total RNA, including microRNA, in high throughput. In addition, Norgen is also pioneering kits for macromolecule fractionation using one column without sample splitting. Towards this goal, Norgen has developed the RNA/DNA/Protein Kit (3-in-1) for purification of all 3 macromolecules from one sample sequentially. In addition, a recent product has been developed that added microRNA fractionation to the RNA/DNA/Protein Kit, termed the All-in-One Purification Kit (4-in-1). These products are unique and novel and offer a substantial cost and time savings over sample splitting and a several-column approach. These powerful and novel tools are designed for researchers who are interested in studying the genome, proteome and transcriptome of a single sample, such as for studies of gene expression including gene silencing experiments or mRNA knock-downs and studies involving biomarker discovery.
2. ProteoSpin™ brand protein kits : For desalting, detergent and endotoxin removal, concentration, inclusion body screening, abundant serum protein depletion, urine protein concentration and on-column peptide preparation for mass spec analysis.
3. DNA kits: For plasmid isolation, PCR purification, gel extraction, eukaryotic and prokaryotic genomic DNA isolation, and front-end sample preparation for diagnostics and forensic applications (including milk bacterial DNA isolation, urine bacterial DNA isolation, saliva DNA isolation, and urine DNA isolation).
4. Molecular weight ladders : A broad range of DNA, RNA and protein ladders are available.
Company Location
Norgen is located in a new, 24,000 square foot state-of-the-art facility in the middle of Niagara region, Ontario, Canada one block from Brock University. We are only 30 minutes from Buffalo and 1 hour from Toron to. Also, Niagara Falls and the wineries of Niagara-on-the-Lake are located within 10 minutes of the facility. The building itself is on a large property adjacent to the 406 Highway in Thorold’s technology district, and the property offers much room for expansion.