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蚂蚁淘在线 / 品牌中心 / MCE / Medchemexpress/G-418 disulfate(Synonyms: Geneticin sulfate; Antibiotic G-418 sulfate)/HY-17561/1g

Medchemexpress/G-418 disulfate(Synonyms: Geneticin sulfate; Antibiotic G-418 sulfate)/HY-17561/1g

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￥1320.00

货号：HY-17561-10mM*1mLinWater

浏览量：127

品牌：MCE

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商品描述

G-418(disulfate)isanaminoglycosideantibioticsimilarinstructuretogentamicinB1,whichblockspolypeptidesynthesisbyinhibitingtheelongationstepinbothprokaryoticandeukaryoticcells.

Description	G-418(disulfate)isanaminoglycosideantibioticsimilarinstructuretogentamicinB1,whichblockspolypeptidesynthesisbyinhibitingtheelongationstepinbothprokaryoticandeukaryoticcells.
InVitro	G418isaninhibitorofmanypro-andeukaryotesatconcentrationsfrom1-300microgram/mL.ResistancetoG418conferringbytheneogenefromTn5encodinganaminoglycoside3"-phosphotransferase,APT3"IIiscommonlyusedinlaboratoryresearchtoselectgeneticallyengineeredcells^[1].Ingeneralforbacteriaandalgaeconcentrationsof5mg/Lorlessareused,formammaliancellsconcentrationsofapproximately400mg/Lareusedforselectionand200mg/Lformaintenance.Resistantclonesselectioncouldrequirefrom1toupto3weeks^[2].
InVivo	G418(40and80mg/kg)forthreeconsecutivedaysaresufficienttoeliminateallnontransfectedT.bruceibruceiparasitesfrominfectedmice^[3].
References	[1].DaviesJ,etal.Anewselectiveagentforeukaryoticcloningvectors.AmJTropMedHyg.1980Sep;29(5Suppl):1089-92. [2].LiY,etal.InhibitoryeffectsofantisenseRNAofHAb18G/CD147oninvasionofhepatocellularcarcinomacellsinvitro.WorldJGastroenterol.2003Oct;9(10):2174-7. [3].MurphyNB,etal.UseofaninvivosystemtodeterminetheG418resistancephenotypeofbloodstream-formTrypanosomabruceibruceitransfectants.AntimicrobAgentsChemother.1993May;37(5):1167-70.

AnimalAdmiNISTration ^[3]	TocharacterizethesensitivityofthetrypanosomepopulationstoG418invivo,bloodstreamformsofT.bruceibruceiGUTat3.1andT.bruceibruceiGUTat3.1/BBR3areexpandedseparatelyinsublethallyirrADIatedmice.Priortothefirstpeakofparasitemia,trypanosomesarecollected,andaliquotscontaining106trypanosomesareinoculatedintraperitoneallyintomice.Twenty-fourhoursfollowinginfection,themicearedividedintogroupsandtreatedwithG418atadoseof10,20,30,40,50,or80mg/kgofbodyweight(bw)byinoculatingintraperitoneally0.2mLofthedruginsterilewater.At24and48hfollowingthefirsttreatment,G418isadministeredtoanimalsineachgroupatthesamedoseasbefore,resultinginthreetreatmentspermouse.RepeateddrugtreatmentsarenecessarytoensurecompleteeliminationofnontransfectedGUTat3.1parasitesfromthemice.Micearethenmonitoreddaily,for33days,forthepresenceofparasitesbymicroscopicexaminationofwet-bloodfilms.Animalsfoundtobeparasitemicarerecordedandthenremovedfromtheexperiment.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.
References	[1].DaviesJ,etal.Anewselectiveagentforeukaryoticcloningvectors.AmJTropMedHyg.1980Sep;29(5Suppl):1089-92. [2].LiY,etal.InhibitoryeffectsofantisenseRNAofHAb18G/CD147oninvasionofhepatocellularcarcinomacellsinvitro.WorldJGastroenterol.2003Oct;9(10):2174-7. [3].MurphyNB,etal.UseofaninvivosystemtodeterminetheG418resistancephenotypeofbloodstream-formTrypanosomabruceibruceitransfectants.AntimicrobAgentsChemother.1993May;37(5):1167-70.

AnimalAdmiNISTration
^[3]

TocharacterizethesensitivityofthetrypanosomepopulationstoG418invivo,bloodstreamformsofT.bruceibruceiGUTat3.1andT.bruceibruceiGUTat3.1/BBR3areexpandedseparatelyinsublethallyirrADIatedmice.Priortothefirstpeakofparasitemia,trypanosomesarecollected,andaliquotscontaining106trypanosomesareinoculatedintraperitoneallyintomice.Twenty-fourhoursfollowinginfection,themicearedividedintogroupsandtreatedwithG418atadoseof10,20,30,40,50,or80mg/kgofbodyweight(bw)byinoculatingintraperitoneally0.2mLofthedruginsterilewater.At24and48hfollowingthefirsttreatment,G418isadministeredtoanimalsineachgroupatthesamedoseasbefore,resultinginthreetreatmentspermouse.RepeateddrugtreatmentsarenecessarytoensurecompleteeliminationofnontransfectedGUTat3.1parasitesfromthemice.Micearethenmonitoreddaily,for33days,forthepresenceofparasitesbymicroscopicexaminationofwet-bloodfilms.Animalsfoundtobeparasitemicarerecordedandthenremovedfromtheexperiment.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

References

[1].DaviesJ,etal.Anewselectiveagentforeukaryoticcloningvectors.AmJTropMedHyg.1980Sep;29(5Suppl):1089-92.
[2].LiY,etal.InhibitoryeffectsofantisenseRNAofHAb18G/CD147oninvasionofhepatocellularcarcinomacellsinvitro.WorldJGastroenterol.2003Oct;9(10):2174-7.
[3].MurphyNB,etal.UseofaninvivosystemtodeterminetheG418resistancephenotypeofbloodstream-formTrypanosomabruceibruceitransfectants.AntimicrobAgentsChemother.1993May;37(5):1167-70.

MolecularWeight

692.71

Formula

C₂₀H₄₄N₄O₁₈S₂

CASNo.

108321-42-2

Storage

Powder	-20°C	3years
	4°C	2years
Insolvent	-80°C	6months
	-20°C	1month

Shipping

RoomtemperatureincontinentalUS;mayvaryelsewhere

Solvent&Solubility

H₂O:≥30mg/mL

*"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">

Purity:>98.0%

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