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HydrogenPeroxideDetection
lumigenHyPerBluChemiluminescentReagentisanovelready-to-usesubstrateforthedirectdetectionofhydrogenperoxide.Reactionofthesubstratewithhydrogenperoxiderapidlygeneratessustainedhigh-intensityluminescenceformaximumdetectionsensitivityinsolutionassays.CoupledwithoxidasestheLumigenHyPerBlusubstratealsoallowsonestepindirectquantitationofoxidasesubstratesortheoxidaseitself.
LumigenHyPerBlusubstrateissuitableforautomationandminiaturizationto384-wellor1536-wellformat.LumigenHyPerBlureagentswidetoleranceforcommonassayadditives(egDMSO),lackofintermediaries,chemicalspecificity,one-componentstructureandexcellentstABIlityleadtoassaysimplicity,excellentrobustnessandunmatchedconvenienceforhighthroughputscreeninglaboratories.
- Directdetectionofperoxide,oxidaseenzymesortheirsubstrates
- Singleready-to-usereagentformulationwithlongstabilityforconvenience
- Broaddynamicrangewithbrightsustainedchemiluminescence
- Excellentsensitivityinsolutionassays
LumigenHyPerBluTechnology
LumigenHyPerBlureagentisanovelchemiluminescentsubstratethatdirectlyreactswithhydrogenperoxide(withoutperoxidase)rapidlygeneratingasustainedhigh-intensitychemiluminescence.ThesignalintensitycanbemeasuredonPMTbasedplatereADIngluminometersorCCDimagingsystems.Longstabilityoftheformulationcombinedwiththewidehydrogenperoxidedynamicrange(>5orders)allowforsensitiveandreproducIBLeassays.


IndirectDetectionofOxidases
LumigenHyPerBluchemiluminescentreagentoffersano-labelenzyme-freetechniqueforthedetectionofoxidaseactivity.BecausetheLumigenHyPerBlusubstratedirectlymeasureshydrogenperoxideproducedfromoxidativereactions,assaysaresignificantlysimpler,moreflexible,andavoidcomplicationsassociatedwithlabel-basedtechnologies.


