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BlottingAPSubstrate
Lumi-PhosPlusisanovel,ready-to-usechemiluminescentreagentformulationcontainingthealkalinephosphatase(AP)substrate,lumigenPPDandapatentedenhancer.Lumi-PhosPlusprovideshighlysensitivechemiluminescentdetectionofAPwithDNAprobesorantibodyconjugatesinsolutionandonsolidsupportssuchasmembranes.Thisreagentisrecommendedforeitherdirectchemiluminescentdetectionofalkalinephosphatase(AP)labelednucleicacidsorindirectlytodetecthapten-labelednucleicacidsprobedwithspecificanti-haptenantibodiesconjugatedwithAPonaSouthernblotmembraneoranyothersolidsurface.
- Excellentsensitivity–singlecopygenedetection
- Highintensitylightproductionwithlowbackground
- Sustainedluminescence-withlowconcentrationsofAP,thelightintensityremainsconstantforseveralhours
- HighstABIlityandlongshelflife
SolutionKinetics
Lumi-PhosPlushasasignificantlyhighersignaltobackgroundratiothanLumi-Phos530duetoitslowerreagentbackground.Lumi-PhosPlusreaches90%ofmaximumlightoutputapproximately27minutesafterintroductionofenzymeinhomogeneousassaysat37°C.Lightemissionismaximalat470nm.




