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蚂蚁淘在线 / 品牌中心 / Anatrace / Anatrace/PurePEGs结晶筛/PP96-1ML/1ML

Anatrace/PurePEGs结晶筛/PP96-1ML/1ML

价格
￥4720.00
货号：PP96-1ML
浏览量：127
品牌：Anatrace
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商品描述
StorageConditions:4-20C
pH:Varies
Conductance:Varies
Appearance:ClearSolutions

Features:

  • 96IndividualCrystallizationConditions
  • UltraPurePEGs-USPGrade
  • PolydispersePEGCocktail-5PEGsineachcondition
  • pHmeasuredandrecordedforboththestockbuffersandfinalcrystallizationconditions
ThePurePEGsCrystallizationScreenfeaturesconditionsthateachcontainamixtureofpurifiedpolyethyleneglycols,rangingfrom300MWto8000MW. Atatotalconcentrationof22.5%PEGcombinedwithvarioussaltsandbuffers,theconditionsaredesignedtospanawidecrosssectionofPEGcrystallizationspace. 

ManysuccessfulcrystallizationconditionscomprisecombinationsofPEGsandsaltsadjustedtoaparticularpH. However,thenecessitytosampleabroadrangeofPEGprecipitantsduringinitialsparsematrixscreeningrenderscomprehensivePEGscreeningatthisstagehighlychallenging. Interestingly,thereisevidencetosuggestthatproteinconcentrationconditionsidentifiedusingaparticularPEGcanberepeatedwhenusingmixturesofPEGsasasingleprecipitant. Consequently,amorerationalscreeningstrategyshouldbetousemixturesofPEGsratherthanconditionscontainingindividualPEGs. InVaporDiffusionexperiments,thisapproachsignificantlyminimizesthenumberofconditionstobesampledandthevolumeofproteinnecessarytoexplorePEGcrystallizationspace.

Citations:
  1. J.Newmanetal.ActaCrystallogrD.61,1426(2005)
  2. R.Pageetal.ActaCrystallogD.59,1028(2003)
  3. J.W.Woohetal.ActaCrystallogD.59,769(2003)
  4. J.T.Bukrinskyetal.JApplCryst.34,533(2001)
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