快报
欢迎光临蚂蚁淘在线！ 请登录 免费注册
商品
品牌
文章
热门搜索：
快报
品牌首页 品牌新闻 品牌简介 产品中心 品牌咨询 联系我们
品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / Kapa biosystems / 含核糖酶（HMR）/KK8560/24文库的Kapa生物系统/Kapa RNA HyperPrep试剂盒

含核糖酶（HMR）/KK8560/24文库的Kapa生物系统/Kapa RNA HyperPrep试剂盒

价格
￥43200.00
货号：KK8560
浏览量：127
品牌：Kapa biosystems
服务
全国联保
正品保证
正规发票
签订合同
商品描述

Description:

KAPARNAHyperPrepwithRiboErase

KAPARNAHyperPrepKitswithRiboErase(HMR)

Single-DayRNA

TheKAPARNAHyperPrepKitswithRiboErase(HMR)utilizenovelchemistrythatenablesthecombinationofenzymaticstepsandfewerreactionpurifications,resultinginatrulystreamlinedsolutionforthepreparationofhigh-qualityrRNA-depletedRNA-seqlibraries.Byutilizingatargetedenzymaticmethodfordepletion,ourworkflowenablesefficientreductionofribosomalRNA(rRNA)andamorecompleterepresentationofthetranscriptome,includingprecursormRNAsandnon-codingRNA(ncRNA).Thestrand-specificworkflowisflexIBLe–supportinglibraryconstructionfromlower-inputamountsanddegradedsamples.KitscontainallreagentsrequiredforrRNAdepletionandlibrarypreparation,withtheexceptionofKAPAAdapters(availableseparately).Benefitsinclude:

  • single-daylibraryconstruction,inclusiveofrRNAdepletion
  • reducedhands-onandoveralltimethroughfewerenzymaticandreactioncleanups
  • flexibleinputof25ng–1µgtotalRNAfromhuman,mouse,orratspecies*
  • highersuccessrateswithlowerinputanddegradedsamples
  • maintainover99%strandspecificity*
  • KAPAPureBeadsincludedforreactionpurifications

NEW!KAPADual-IndexedAdapterKitsarenowavailable. FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.DownloadourAdapterandBeadCalculator

 

*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

The KAPA RNA Hyper Prep Kit with RiboErase (HMR) results in a reduction in the total number of reads wasted due to both PCR duplicates and alignment to rRNA loci in comparison to the TruSeq and NEBNext kits. Libraries were generated in quadruplicate with 25 ng of high-quality Universal Human Reference (UHR) RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.
Better utilize sequencing capacity. (B) Libraries were generated in quadruplicate with 25 ng (rRNA depletion) and 50 ng (mRNA capture) of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Data on file.
PrevNext

Sequencewhatmatters

  • WastefewerreadsduetothecombinationofrRNAcarryoverandPCRduplicates
  • Identifymoreuniquetranscriptsandgeneswithequivalentsequencing
Improved coverage uniformity. For the top 1000 transcripts, the KAPA RNA HyperPrep workflows result in more even coverage across transcript lengths, as assessed by both a normalized coverage plot and coverage coefficient of variation (CV), in comparison to competitor workflows. (A) Libraries were generated with 25 ng (rRNA depletion) and 50 ng (mRNA capture) of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Data on file.
Improved coverage uniformity. For the top 1000 transcripts, the KAPA RNA HyperPrep workflows result in more even coverage across transcript lengths, as assessed by both a normalized coverage plot and coverage coefficient of variation (CV), in comparison to competitor workflows. (B) Improved coverage of GC-rich regions (outlined in red) of the YBX1 (rRNA depletion) and SLC2A4RG (mRNA capture) genes was demonstrated using the Kapa workflows. Data on file.
PrevNext

Achievesuperiorcoverageuniformity

  • Obtainmoreuniformdistributionofreadsacrosstranscripts
  • ImprovecoverageofdifficultGC-richtranscripts
Identify more genes and transcripts. With equivalent sequencing, the KAPA RNA HyperPrep Kit with RiboErase (HMR) in comparison to competitor workflows identified more genes and transcripts. Data on file.
PrevNext

Generatehigh-qualitylibrariesfromdegradedsamples

  • Inputaslittleas25ngwithFFPEsamples,dependingontotalRNAquality
  • Achievelowduplicationratesandhighlyefficient,reproduciblerRNAremovalwithdegradedsamples
  • Identifymoreuniquetranscriptsandgeneswithequivalentsequencing
High-level of agreement between paired FFPE and fresh frozen expression data, in transcripts per million (TPM). Pearson correlation coefficients show a higher degree of agreement with the KAPA RNA Hyper Prep Kit with RiboErase in comparison to the TruSeq Stranded Total RNA Library Prep with Ribo-zero Gold and NEBNext Ultra Directional Library Preparation with the rRNA Depletion Kit. Libraries were generated in duplicate using 100 ng inputs of paired FFPE-derived and fresh frozen breast tumor total RNA samples using the manufacturers’ standard recommendations per workflow. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.
High-level of agreement between paired FFPE and fresh frozen expression data, in transcripts per million (TPM). Pearson correlation coefficients show a higher degree of agreement with the KAPA RNA Hyper Prep Kit with RiboErase in comparison to the TruSeq Stranded Total RNA Library Prep with Ribo-zero Gold and NEBNext Ultra Directional Library Preparation with the rRNA Depletion Kit. Libraries were generated in duplicate using 100 ng inputs of paired FFPE-derived and fresh frozen breast tumor total RNA samples using the manufacturers’ standard recommendations per workflow. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.
High-level of agreement between paired FFPE and fresh frozen expression data, in transcripts per million (TPM). Pearson correlation coefficients show a higher degree of agreement with the KAPA RNA Hyper Prep Kit with RiboErase in comparison to the TruSeq Stranded Total RNA Library Prep with Ribo-zero Gold and NEBNext Ultra Directional Library Preparation with the rRNA Depletion Kit. Libraries were generated in duplicate using 100 ng inputs of paired FFPE-derived and fresh frozen breast tumor total RNA samples using the manufacturers’ standard recommendations per workflow. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.
PrevNext

Achievereliableresultswithdegradedinputs

  • Attainahigh-degreeofexpressioncorrelationbetweenpairedFFPEandfreshfrozensampleswhichprovidesincreasedconfidenceinsequencedataaccuracy

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPA mRNA HyperPrep Kits

KAPAmRNAHyperPrepKits

KAPA Stranded mRNA-Seq Kits

KAPAStrandedmRNA-SeqKits

Library Amplification

KAPALibraryAmplificationKits

Library Preparation

KAPAHyperPrepKits

Library Quantification

KAPALibraryQuantificationKits

Applications:

Applications
  • Wholetranscriptome
  • Geneexpressionanalysis
  • Detectionofgenefusions,isoforms,andotherstructuralvariants
  • Noveltranscriptidentification,includingnoncodingtranscripts
  • SNVdiscovery

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

EnzymesandbuffersforrRNAdepletion,CDNAsynthesis,andlibrarypreparationcanbestoredforupto10monthsat-20°C.KAPAPureBeadscanbestoredforupto10monthsat4°C.(USonly)

KitscontainHybridizationBuffer,HybridizationOligos(HMR),DepletionBuffer,RNaseH,DNaseBuffer,DNase,Fragment,PrimeandEluteBuffer(2X),1stStrandSynthesisBuffer,KAPAScript,2ndMarkingBuffer,2ndStrandSynthesis&A-TailingEnzymeMix,LigationBuffer,DNALigase,PEG/NaClSolution,KAPAPureBeads,LibraryAmplificationPrimerMix(10X),andKAPAHiFiHotStartReadyMix(2X).

RNAHyperRibo_Component Chart

Specifications

Spec
Description
CompatiblePlatform
0616
LibraryType
RNA
StartingMaterial
High-qualityanddegradedtotalRNA
InputAmount
25ng–1µg
Kapa是世界上著名的生物技术公司。总部位于美国波士顿, 公司的主要理念是运用分子和遗传学技术来打造新一代的生物科学和医学诊断产品。Kapa公司目前主要是在“直接进化（directed evolution technology platform）”的技术平台上来筛选和生产高品质的生物酶。目前生物医药领域所使用的大部分生物酶都只是天然存在形式，即使有些酶经过基因工程改造，但是由于酶分子自身的结构和功能限制，要想得到真正满足科研工作人员需要的产品，却是一件费时费力的事情，而且常常达不到预期的要求。“直接进化”技术正是针对这一现实情况，运用遗传，基因工程，生物信息学等技术来在分子水平上设计真正满足不同实际要求的生物酶。通过创新，Kapa为广大的科研人员提供了一系列拥有多项技术的产品，不仅能够满足普通科研需要，同时为高通量，基因芯片等高新技术的普及创造了条件。 运用“直接进化”技术，Kapa目前已经开发

  • 资质认证

    获得国家资质，权威认证！

  • 全国联保

    全国联保，官方无忧售后

  • 正规发票

    正规发票，放心购买

  • 签订合同

    签订合同，保障您的权益

购物指南
购物流程
注册登录
账户管理
订单查看
常见问题
FAQS
蚁豆兑换
物流说明
评论规则
发票说明
价格说明
支付说明
服务协议
版权声明
诚信保障
免责申明
销售协议
隐私条款
法律申明
售后服务
退换货政策
工作时间
退款说明
售后支持
售后申请表模板列表
关于我们
公司简介
联系我们
资质证书
人才招聘
商务合作
万众创业
网址导航
蚂蚁淘商城
科研狗
苏州蚂蚁淘
生物信息网
现货促销

微信公众号

copyright©2005-2021 蚂蚁淘在线版权所有　苏州蚂蚁淘生物科技有限公司 www.assay-online.com ICP号：苏ICP备17049038号-16

/**/