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蚂蚁淘在线 / 品牌中心 / Kapa biosystems / Kapa生物系统/Kapa mRNA HyperPrep试剂盒/KK8540/24文库

Kapa生物系统/Kapa mRNA HyperPrep试剂盒/KK8540/24文库

价格
￥21600.00
货号：KK8540
浏览量：127
品牌：Kapa biosystems
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商品描述

Description:

KAPARNAHyperPrepKit

KAPAmRNAHyperPrepKits

Single-DayRNA

TheKAPAmRNAHyperPrepKitsutilizenovelchemistrythatenablesthecombinationofenzymaticstepsandfewerreactionpurifications,resultinginatrulystreamlinedsolutionforthepreparationofhigh-qualitymRNA-seqlibraries.mRNAcapturebeadsareusedpriortolibrarypreparation,whichenrichesformaturemRNAovernon-polyadenylatedspecies,suchasribosomal,precursor,andnoncodingRNAs.

Thestrand-specificworkflowisflexIBLe–supportinglibraryconstructionfromlower-inputamountsanddegradedsamples.KitscontainallreagentsrequiredformRNAcaptureandlibrarypreparation,withtheexceptionofKAPAAdapters(availableseparately).Benefitsinclude:

  • single-daylibraryconstruction,inclusiveofmRNAcapture
  • reducedhands-onandoveralltimethroughfewerenzymaticandreactioncleanups
  • flexibleinputof50ng–1µgtotalRNA*
  • maintainover99%strandspecificity*
  • KAPAPureBeadsincludedforreactionpurifications

KAPARNAHyperPrepKits

KAPARNAHyperPrepKitsleveragethesameCDNAsynthesisandlibrarypreparationworkflowimprovementsasoutlinedfortheKAPAmRNAHyperPrepKits.KAPARNAHyperPrepKitsincludeallenzymesandbuffersrequiredforcDNAlibrarypreparation,butdonotcontainmRNAcapturereagentsorKAPAAdapters.Kitscanbeusedtopreparelibrariesfrom1ng–100ngoftotal,poly(A)-selected,orrRNA-depletedRNA.

RNA-Hyper_Workflow

NEW!KAPADual-IndexedAdapterKitsarenowavailable. FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapter andBeadCalculator.

DownloadourAdapterandBeadCalculator

 

*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

The KAPA mRNA HyperPrep Kit results in a reduction in the total number of reads wasted due to both PCR duplicates and alignment to rRNA loci in comparison to the TruSeq and NEBNext kits. Libraries were generated in quadruplicate with 50 ng of high-quality Universal Human Reference (UHR) RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.
With an equivalent amount of sequencing, more genes and unique transcripts are identified using the KAPA mRNA HyperPrep Kit in comparison to the TruSeq and NEBNext kits. Libraries were generated in quadruplicate with 50 ng of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.
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Sequencewhatmatters

  • WastefewerreadsduetothecombinationofrRNAcarryoverandPCRduplicates
  • Identifymoreuniquetranscriptsandgeneswithequivalentsequencing
For the top 1000 transcripts, the KAPA mRNA Hyper Prep Kit results in more even coverage across transcript lengths, as assessed by both a normalized coverage plot and coverage coefficient of variation (CV), in comparison to competitor workflows. Libraries were generated with 50 ng of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.
Improved coverage of GC-rich regions (outlined in red) of the YBX1 (rRNA depletion) and SLC2A4RG (mRNA capture) genes, along with improved splice junction and antisense transcript detection was demonstrated using the KAPA mRNA Hyper Prep Kit in comparison to competitor workflows. Libraries were generated with 50 ng of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.
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Achievesuperiorcoverageuniformity

  • Obtainmoreuniformdistributionofreadsacrosstranscripts
  • ImprovecoverageofdifficultGC-richtranscripts

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPA RNA HyperPrep Kits with RiboErase

KAPARNAHyperPrepKitswithRiboErase

KAPA Stranded mRNA-Seq Kits

KAPAStrandedmRNA-SeqKits

Library Amplification

KAPALibraryAmplificationKits

Library Preparation

KAPAHyperPrepKits

Library Quantification

KAPALibraryQuantificationKits

Applications:

Applications
  • Geneexpressionanalysis
  • Detectionofgenefusions,isoforms,andotherstructuralvariants
  • Noveltranscriptidentification,includingnoncodingtranscripts
  • SNVdiscovery
  • Wholetranscriptome(KAPARNAHyperPrepKitsonly)
  • Targetedtranscriptome(KAPARNAHyperPrepKitsonly)

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

EnzymesandbuffersforrRNAdepletion,cDNAsynthesis,andlibrarypreparationcanbestoredforupto10monthsat-20°C.mRNAcapturereagentsandKAPAPureBeadscanbestoredforupto10monthsat4°C.(USonly)

KitsincludereagentsforRNAfragmentation,cDNAsynthesis,andlibrarypreparation.KitswithreagentsformRNAcapturearealsoavailable.

RNAHyper_Component Chart

Specifications

Spec
Description
CompatibilityPlatform
IlluminaHiSeq,NextSeq,andMiSeq
LibraryType
RNA
StartingMaterial
RNAHyperPrepKit:High-qualitytotalRNA,mRNA,orrRNA-depletedRNA;mRNAHyperPrepKit:High-qualitytotalRNA
StartingMaterial
RNAHyperPrepKit:1ng–100ng;mRNAHyperPrepKit:25ng–1µg
Kapa是世界上著名的生物技术公司。总部位于美国波士顿, 公司的主要理念是运用分子和遗传学技术来打造新一代的生物科学和医学诊断产品。Kapa公司目前主要是在“直接进化（directed evolution technology platform）”的技术平台上来筛选和生产高品质的生物酶。目前生物医药领域所使用的大部分生物酶都只是天然存在形式，即使有些酶经过基因工程改造，但是由于酶分子自身的结构和功能限制，要想得到真正满足科研工作人员需要的产品，却是一件费时费力的事情，而且常常达不到预期的要求。“直接进化”技术正是针对这一现实情况，运用遗传，基因工程，生物信息学等技术来在分子水平上设计真正满足不同实际要求的生物酶。通过创新，Kapa为广大的科研人员提供了一系列拥有多项技术的产品，不仅能够满足普通科研需要，同时为高通量，基因芯片等高新技术的普及创造了条件。 运用“直接进化”技术，Kapa目前已经开发