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蚂蚁淘在线 / 品牌中心 / Kapa biosystems / Kapa生物系统/Kapa链RNA序列，带核糖酶/KK8712/12适配器，每个适配器x 40µl

Kapa生物系统/Kapa链RNA序列，带核糖酶/KK8712/12适配器，每个适配器x 40µl

价格
￥9600.00
货号：KK8712
浏览量：127
品牌：Kapa biosystems
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商品描述

Description:

KAPASingle-IndexedAdapterKit,SetB(1.5µM)

KAPAStrandedRNA-SeqwithRiboErase

Evolvedtofocus.

TheKAPAStrandedRNA-SeqKitwithRiboEraseoffersahigh-quality,comprehensivesolutionfortranscriptomesequencing.Byutilizingatargetedenzymaticmethodfordepletion,ourworkflowenablessuperiorreductionofribosomalRNA(rRNA)andamorecompleterepresentationofthetranscriptome,includingprecursormRNAsandnon-codingRNA(ncRNA).KitsalsocontainKAPAHiFiforhigh-efficiencyandlow-biaslibraryamplification,andincludeastreamlined,“with-bead”protocol.

  • Upto99.98%rRNAdepletion*
  • FlexIBLeinputof100ng–1μgtotalRNAfromhuman,mouse,orratspecies*
  • Robustandreproducibleacrossvariousinputamounts
  • Automation-friendlyworkflow

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

DownloadourAdapterandBeadCalculator

 

*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

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IndustryleADIngrRNAdepletion

  • SuperiorrRNAdepletionfromhigh-qualityandFFPEsamples*
  • MoreeconomicalNGSsequencingduetodecreasedrRNAreads,providingdeepersequencingoftranscriptsofinterest
High-quality sequencing data obtained from KAPA Stranded RNA-Seq Kit with RiboErase when compared to the equivalent Illumina or NEB kits. With equivalent mapping rates, KAPA Stranded RNA-Seq Kit with RiboErase provides lower duplication rates, better coverage uniformity and consistently detects more unique transcripts and genes. This enables a more sensitive detection of the expressed genes within the sample. Data on file.
Improved coverage of GC-rich transcripts. IGV image showing coverage and splice junction tracks of the haemoglobin alpha transcript (HBA1) which has a GC content of 62.8%. The GC content track shows GC-rich regions in red, and AT-rich regions in blue. More even coverage, improved splice-site recognition, and more representative plot compared to the undepleted sample (red) is seen when libraries are prepared with the KAPA Stranded RNA-Seq Kit with RiboErase (green) compared to the equivalent Illumina (orange) or NEB (blue) kits. Data on file.
Better detection of low-abundance transcripts. IGV image showing coverage and splice junction tracks of MALAT1, a low abundance long non-coding RNA transcript, which is more comprehensively covered with use of KAPA Stranded RNA-Seq Kit with RiboErase (green) for library preparation. In comparison, Illumina Ribo-Zero Gold (orange) and NEBNext (blue) show less even coverage of this transcript. Data on file.
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Unsurpassedsequencingdataquality

  • Detectionofmoregenesanduniquetranscripts
  • Accurateandclearidentificationofsplicesitesandalternativegenesplicing
  • ImprovedcoverageenablingbetterdetectionofdifficultandGC-richtranscripts
Hierarchically clustered heatmap showing the correlations of gene expression levels between different laboratories and RNA input amounts. All correlations were greater than 0.93, with technical replicates produced in the same lab exceeding 0.98. Generally, all libraries from a single lab, including those produced from different input amounts, clustered together. The data indicates that the KAPA Stranded RNA-Seq Kit with RiboErase delivers robust, reproducible, and unbiased gene expression data from a wide range of input amounts. Data on file.
0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">Correlation of gene expression levels between replicate samples. Libraries prepared using 100 ng of UHR Total RNA with the KAPA Stranded RNA-Seq Kit with RiboErase show a very high correlation (R2>0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.
0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">Correlation of gene expression levels between replicate samples. Libraries prepared using 100 ng of UHR Total RNA with the KAPA Stranded RNA-Seq Kit with RiboErase show a very high correlation (R2>0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.
0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">Correlation of gene expression levels between replicate samples. Libraries prepared using 100 ng of UHR Total RNA with the KAPA Stranded RNA-Seq Kit with RiboErase show a very high correlation (R2>0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.
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Highlyreproduciblesequencingresults

  • Highcorrelationevenbetweendifferenttestingconditions
  • Lowsample-to-samplevariationformorereliableresults
Improved coverage of the whole transcript. The housekeeping gene Beta-Actin has improved coverage at the 3’ and 5’ regions using the KAPA Stranded RNA-Seq Kit with RiboErase (green) when compared to the equivalent Illumina Ribo-Zero Gold (orange) and NEBNext (blue) kits. Data on file.
Minimal positional bias. With 100 ng of high-quality RNA input, 3
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Improvedcoverageuniformity

  • Uniformdistributionofreadsovereachtranscript
  • Minimal5’–3’biasacrosstranscripts


*Dataonfile.

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPA Stranded mRNA-Seq Kits

KAPAStrandedmRNA-SeqKits

KAPA RNA HyperPrep Kits with RiboErase

KAPARNAHyperPrepKitswithRiboErase

KAPA mRNA HyperPrep Kits

KAPAmRNAHyperPrepKits

Library Amplification

KAPALibraryAmplificationKits

Library Preparation

KAPAHyperPrepKits

Library Quantification

KAPALibraryQuantificationKits

Applications:

Applications
  • WholeTranscriptome
  • GeneExpression
  • TranscriptDiscoveryandAnnotation
  • TotalRNA-Seq

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto10monthsat-20ºC.

KitscontainKAPAHybridizationBuffer,KAPAHybridizationOligos(Human/Mouse/Rat),KAPADepletionBuffer,KAPARNaseH,KAPADNaseBuffer,KAPADnase,KAPAFragment,PrimeandEluteBuffer(2X),KAPA1stStrandSynthesisBuffer,KAPAScript,KAPA2ndStrandMarkingBuffer,KAPA2ndStrandSynthesisEnzymeMix,KAPAA-TailingBuffer(10X),KAPAA-TailingEnzyme,KAPALigationBuffer(5X),KAPADNALigase,KAPAPEG/NaClSPRISolution,KAPALibraryAmplificationPrimerMix(10X)andKAPAHiFiHotStartReadyMix(2X).

Components

RIBO-Component Chart

Specifications

Spec
Description
CompatiblePlatform
IlluminaHiSeq®,NextSeq®andMiSeq®
LibraryType
RNA
StartingMaterial
High-qualityTotalRNA
InputAmount
100ng-1µg
Kapa是世界上著名的生物技术公司。总部位于美国波士顿, 公司的主要理念是运用分子和遗传学技术来打造新一代的生物科学和医学诊断产品。Kapa公司目前主要是在“直接进化（directed evolution technology platform）”的技术平台上来筛选和生产高品质的生物酶。目前生物医药领域所使用的大部分生物酶都只是天然存在形式，即使有些酶经过基因工程改造，但是由于酶分子自身的结构和功能限制，要想得到真正满足科研工作人员需要的产品，却是一件费时费力的事情，而且常常达不到预期的要求。“直接进化”技术正是针对这一现实情况，运用遗传，基因工程，生物信息学等技术来在分子水平上设计真正满足不同实际要求的生物酶。通过创新，Kapa为广大的科研人员提供了一系列拥有多项技术的产品，不仅能够满足普通科研需要，同时为高通量，基因芯片等高新技术的普及创造了条件。 运用“直接进化”技术，Kapa目前已经开发