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蚂蚁淘在线 / 品牌中心 / Kapa biosystems / Kapa生物系统/Kapa HyperPlus试剂盒/KK8515/96反应

Kapa生物系统/Kapa HyperPlus试剂盒/KK8515/96反应

价格
￥59520.00
货号：KK8515
浏览量：127
品牌：Kapa biosystems
服务
全国联保
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商品描述

Description:

PCR-Free

KAPAHyperPlusKits

NGSlibrarypreparation. Evolved.

TheKAPAHyperPluskitprovidesastreamlinedworkflowthatincludesfragmentationandlibrarypreparationinasingletube.Buildingonindustry-leADInglibraryconstructionefficiencies,thisintegratedsolutioncombinesenzymaticfragmentation,similarinqualitytomechanicalshearing,withthespeedandconvenienceoftagmentation-basedworkflows.Benefitsinclude:

  • DNAfragmentationandlibraryprepin2.5hours*
  • FlexIBLeDNAsampleinputfrom1ng–1µg*
  • Reducedbiasandmaximizesequencecoverage*
  • PCR-freeworkflows

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

Downloadour AdapterandBeadCalculator

 

*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

Workflow

KAPAHyperPlusWorkflow

ProductHighlights

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Tunableandreproduciblefragmentation

  • Adjustlibraryinsertsizesfrom150–800bpbyvaryingfragmentationtime
  • ReproducibleinsertsizesacrossarangeofGCcontentandDNAinputamounts

Industryleadinglibraryyields…

  • Routinelyachieveconversionrates>80%from≥100nginput*
  • SuperiorperformanceacrossarangeofDNAinputamounts*
  • HighlibraryyieldsenablePCR-freeworkflowsfromaslittleas50ngstartingmaterial*
Single nucleotide polymorphism (SNP) detection sensitivity indicates better sequencing quality. Libraries were prepared for capture using the Nimblegen SeqCap EZ HGSC VCRome panel, 50 ng input, standard capture protocol. SNPs were called using LoFreq with default settings and a minimum per-base depth of coverage cut-off of 10. Sensitivity (true positive rate) was calculated as the ratio of true positives divided by the sum of true positives and false negatives. Total SNPs analysed was greater than 25,000. Data on file.
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…Enablesuperiorsequencingresults

  • Higherconversionratesresultsinfeweramplificationcyclesandlowerduplicationrates
  • Detectlow-frequencymutationswithhighconfidenceduetogreaterlibrarydiversityandmoreuniformsequencecoverage
GC bias comparison. GC bias for C. difficile, E. coli, B. pertussis was assessed by calculating the GC content of the reference in 100 bp bins and plotting normalized coverage across these bins for Covaris and Hyper Prep, HyperPlus, NEBNext ® Ultra ™ and Nextera ™ workflows, using Picard CollectGCBiasMetrics. Libraries were prepared from 1 ng of input DNA. In the absence of sequencing bias, all bins would be equally represented, indicated by a horizontal distribution centered on a normalized coverage of 1.0. Distribution of GC content in the genome is indicated by the grey histograms. Data on file.
GC bias comparison. GC bias for C. difficile, E. coli, B. pertussis was assessed by calculating the GC content of the reference in 100 bp bins and plotting normalized coverage across these bins for Covaris and Hyper Prep, HyperPlus, NEBNext ® Ultra ™ and Nextera ™ workflows, using Picard CollectGCBiasMetrics. Libraries were prepared from 1 ng of input DNA. In the absence of sequencing bias, all bins would be equally represented, indicated by a horizontal distribution centered on a normalized coverage of 1.0. Distribution of GC content in the genome is indicated by the grey histograms. Data on file.
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Minimalsequencecoveragebias

  • Lowersequencebiaswhencomparedtotagmentationandotherenzymaticfragmentationmethods*
  • Equivalentperformancetomechanicalshearing*
  • Lessbiasleadstomoreuniformsequencingcoverageandreducedsequencingcosts*

 

*Dataonfile.

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

Sample QC

KAPAhgDNAQuantificationandQCKit

Library Amplification

KAPALibraryAmplificationKits

Library Quantification

KAPALibraryQuantificationKits

KAPA Stranded mRNA-Seq Kits

KAPAStrandedmRNA-SeqKits

Applications:

Applications
  • WholeGenomeSequencing
  • WholeExomeSequencing
  • TargetedSequencing(custompanels)
  • FFPESampleSequencing

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto6monthsat-20ºC.

KitscontainKAPAFragEnzyme,KAPAFragBuffer(10X),KAPAFragConditioningSolution,KAPAEndRepair&A-TailingBuffer, KAPAEndRepair&A-TailingEnzyme, KAPALigationBuffer, KAPADNALigase, KAPAHiFiHotStartReadyMixand KAPALibraryAmplificationPrimerMix.

 

 

Specifications

Spec
Description
CompatiblePlatform
lluminaHiSeq®,MiSeq®,NextSeq®
LibraryType
DNA
StartingMaterial
genomicDNA,FFPEDNA,PCRamplicons
InputAmount
1ng–1µg
Kapa是世界上著名的生物技术公司。总部位于美国波士顿, 公司的主要理念是运用分子和遗传学技术来打造新一代的生物科学和医学诊断产品。Kapa公司目前主要是在“直接进化（directed evolution technology platform）”的技术平台上来筛选和生产高品质的生物酶。目前生物医药领域所使用的大部分生物酶都只是天然存在形式，即使有些酶经过基因工程改造，但是由于酶分子自身的结构和功能限制，要想得到真正满足科研工作人员需要的产品，却是一件费时费力的事情，而且常常达不到预期的要求。“直接进化”技术正是针对这一现实情况，运用遗传，基因工程，生物信息学等技术来在分子水平上设计真正满足不同实际要求的生物酶。通过创新，Kapa为广大的科研人员提供了一系列拥有多项技术的产品，不仅能够满足普通科研需要，同时为高通量，基因芯片等高新技术的普及创造了条件。 运用“直接进化”技术，Kapa目前已经开发