RubiscoQuantitationKit

ProductInformation

KitContains:

• RabbitpolyclonalantibodytoRbcL(100µg)

• Rubisco(RbcL)recombinantproteinstandard(250µl)

• PEBextractionbuffer(3x2ml)(4Xstock)

Storage(AntibodyandStandard)

• lyophilized/reconstitutedat-20°C(6months)or-80°C(longtermstorage/years). Pleaseavoidfreezingandthawingofreconstitutedantibodies. Makealiquotsinstead

Storage(Buffer)

• StableatRTforatleast1month;(shorttermstorage/month)at4°Corlongtermstorage(1year)at20°C.

Background:

• TherubiscoquantitationkitissuitableforquantitationofRubiscoinplantandalgalsamplesusingquantitativeimmunoblotting. Thekitcontainsanti-RbcLantibody,recombinantRbcLproteinandPEBbufferforquantitativeproteinextraction.

ApplicationInformation

• Westernblot1:5,000-10,000withstandardECL
• Expected/apparentMW[kDa]:52.7kDa(ArABIdopsisthaliana),52.5kDa(cyanobacteria),52.3(Chlamydomonasreinhardtii)
• Confirmedreactivity:Arabidopsisthaliana,Chlamydomonasreinhardtii,Cyanophoraparadoxa,Emilianahuxleyi,Euglenagracilis,Gonyaulaxpolyedra,Heterosigmaakashiwo,Micromonaspusila,Porphyrasp.Spinaciaoleracea,SynechococcusPCC7942,Thalassiosirapseudonana
• Predictedreactivity:diandmonocots,conifers,mosses,liverworts,welwitschia,greenalgae,redalge,brownalgae,cryptomonad,cyanobacteriaincludingprochlorophytes,gamma-proeobacteria,beta-proteobacteria,alphaproteobacteria
• Notreactivein:noconfirmedexceptinfrompredictedreactivityknowninthemoment
• RbcLproteinstandard:at0.15pmoles/µl,amountenoughforgenerationofstandardcurvein85assays(standardcurve:0.0625pmoles,0.125pmoles,0.25pmoles)
• PEBextractionbuffer:amountenoughtfor48isolationsofplantmaterial(using500µl1xPEBfor100mgfreshweight)or120isolationsofalgalmaterial(using200µl1xPEBforcellamountscorrespondingto4-10ugtotalchlorophyll)

 

• Storage
  :-20°C
• BrandName
  :bioPLUS
• Size
  :1Kit
• CatalogNo
  :30210005-1

Fungigone™，真菌污染抑制剂正常价格SKU# 40600006-1FungiGone™ 是 bioWORLD 专有的抗真菌试剂配方该产品可显着减少组织培养应用中真菌的生长，而不会影响植物组织。非常适合用于营养繁殖和种子萌发测试的组织外植体。以 100X 浓缩液形式提供体外植物组织培养测试技术规格外观形式：液体保质期：1年储存温度：2-8°C危险品运输：无害