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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description
TrypLE™Expressisananimalorigin-free,recombinantenzymeusedfordissociatingawiderangeofadherentmammaliancells,includingCHO,HEK293,A529,primaryhumankeratinocytes,andembryonicstemcells.TrypLE™ExpresscleavespeptidebondsontheC-terminalsidesoflysineandarginine,andisadirectreplacementfortrypsin.Itsexceptionalpurityincreasesspecificityandreducesdamagetocellsthatcanbecausedbyotherenzymespresentinsometrypsinextracts.WeofferavarietyofTrypLE™formulationsforarangeofcellcultureapplications.
Comparedtotrypsinandotherdissociationreagents,Gibco®TrypLE™Expressis:
Gentleoncells
•Room-temperaturestable
•Easytouse
•Animalorigin-free(AOF)
ThisTrypLE™Expressismodifiedasfollows:
| With |
•PhenolRed |
•EDTA |
TheformulationofTrypLE™solutionsisavailable,althoughwekeeptheenzymeconcentrationconfidential.
GentleOnCells
TrypLE™Express"exceptionalpurityincreasesspecificityduetotheactionofasingleenzyme.Thisreducesdamagecausedbycleavagefromenzymespresentinsometrypsinpreparationsandinotherproteaseextracts.
Room-TemperatureStable
TrypLE™Expressisroom-temperaturestableandreadytouse.TrypLE™Expressremainsstablefor24monthsatroomtemperatureor2–8°C,makingstorageandhandlingeasyandconvenient.
EasytoUse
TrypLE™Expresscanbedirectlysubstitutedfortrypsininexistingprotocols.Inaddition,dilutionaloneinactivatesTrypLE™Express,avoidingtheneedfortrypsininhibitors,suchasFBS.
AnimalOrigin-Free(AOF)
Unlikeporcinetrypsin,TrypLE™Expressisfreeofanimal-derivedcomponents.
cGMPManufacturingandQualitySystem
Forsupplychaincontinuity,wemanufactureGibco®TrypLE™ExpressattwoseparatefacilitieslocatedinGrandIsland,NYandScotland,UK.BothsitesarecompliantwithcGMPmanufacturingrequirements,arecertifiedtotheISO13485standard,andareregisteredwiththeFDAasmedicaldevicemanufacturers.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
Specifications
| Chelators: | EDTA |
|---|---|
| PhenolRedIndicator: | PhenolRed |
| Classification: | AnimalOrigin-Free |
| EnzymeStABIlity: | RoomTemperatureStable |
| Concentrated: | 1X |
| Form: | Liquid |
| Osmolality: | CheckandRecord |
| ProductLine: | Gibco® |
| ProductSize: | 100mL |
| ReagentType: | TrypsinReplacement |
| TestsPerformed: | InVitroBioassay |
| pHRange: | CheckandRecord |
| GreenFeatures: | Energyefficient, Sustainablepackaging |
| ShippingCondition: | RoomTemperature |
Contents&storage
Storageconditions:15°Cto30°C,protectfromlight
Alternatestorageconditions:2°Cto8°Cor-5°Cto-20°C
Shippingconditions:Roomtemperature
Shelflife:24monthsfromdateofmanufacture
Alternatestorageconditions:2°Cto8°Cor-5°Cto-20°C
Shippingconditions:Roomtemperature
Shelflife:24monthsfromdateofmanufacture
Mediaformulations
TrypLE™Express(1X),phenolred
