Description

DMEM (Dulbeccos Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool. This DMEM is modified as follows:

With	Without
• Low Glucose	• HEPES
• L-Glutamine
• Sodium Pyruvate
• Phenol Red

The complete formulation is available.Using DMEMDMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagles Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.Product useFor human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco® DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).cGMP manufacturing and quality systemDMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM product made in our Scotland facility (31885-023). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.For In Vitro Diagnostic Use.

Specifications

Glutamine:Phenol Red Indicator:Form:Glucose:Serum Supplementation:HEPES Buffer:Sodium Pyruvate Additive:Product Line:Product Size:Volume (Metric):Green Features:Shipping Condition:

L-Glutamine

Phenol Red

Liquid

Low Glucose

Standard Serum Supplementation

No HEPES

Sodium Pyruvate

Gibco®

1,000 mL

1000 ml

Sustainable packaging

Room Temperature

Contents & storage

Storage conditions:2-8° C.Protect from lightShipping conditions: AmbientShelf life: 12 months from date of manufacture

Media formulations

11885 - DMEM, low glucose, pyruvate

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