Description:

IL-4 specific spots produced by 2x10E4 human PBMC.
Stimulation: PMA/ionomycin.

Plate characteristics:

Transparent

Contents:

• Coating antibodies
• Biotinylated detector antibodies
• Phi-labeled goat anti-biotin antibodies
• Activator solutions
• Blocking stock solution
• Dilution buffer T
• Tween-20
• Transparent ELISPOT plates with lids and cover slips
• Detailed manual

References list:

• Autoreactive T cell responses show proinflammatory polarization in diabetes but a regulatory phenotype in health. Arif, Sefina, et al. J. Clin. Invest. 2004 Feb;113: 451-63
• Immunological efficacy of heat shock protein 60 peptide DiaPep277 therapy in clinical type I diabetes. Huurman, V A L, et al. Clin. Exp. Immunol. 2008 Jun;152: 488-97
• Development of type 1 diabetes despite severe hereditary B-lymphocyte deficiency. Martin, S, et al. N. Engl. J. Med. 2001 Oct 4;345: 1036-40
• Beta-cell function in new-onset type 1 diabetes and immunomodulation with a heat-shock protein peptide (DiaPep277): a randomised, double-blind, phase II trial. Raz, I, et al. Lancet 2001 Nov 24;358: 1749-53
• Comparison of cytokine ELISpot assay formats for the detection of islet antigen autoreactive T cells. Report of the third immunology of diabetes society T-cell workshop. Schloot, Nanette C, et al. J. Autoimmun. 2003 Dec;21: 365-76
• Analysis of anthrax and plague biowarfare vaccine interactions with human monocyte-derived dendritic cells. Skowera, Anna, et al. J. Immunol. 2005 Dec 1;175: 7235-43
• FTY720 treatment of kidney transplant patients: a differential effect on B cells, naïve T cells, memory T cells and NK cells. Vaessen, Leonard M B, et al. Transpl. Immunol. 2006 Apr;15: 281-8
• Lack of anti-tumour reactivity despite enhanced numbers of circulating natural killer T cells in two patients with metastatic renal cell carcinoma. Vyth-Dreese, F A, et al. Clin. Exp. Immunol. 2010 Dec;162: 447-59
• Human mesenchymal stem cells modulate cellular immune response to islet antigen glutamic acid decarboxylase in type 1 diabetes. Zanone, Maria M, et al. J. Clin. Endocrinol. Metab. 2010 Aug;95: 3788-97
• Anti-CD25 therapy impairs donor-specific Th1 and Th2 cytokine-producing peripheral blood cells after clinical heart transplantation. van Besouw, N M, et al. Transplant. Proc. 2002 Nov;34: 2942-3

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