Description:

Smad transcription factors lie at the center of the transforming growth factor-beta (TGF-β) pathway, which is one of the most important cytokine signaling pathways. Members of the transforming growth factor-beta (TGF-β) superfamily bind to serine/threonine kinase receptors and specifically activate intracellular Smad proteins. Smads 2 and 3 are activated by activin/nodal and TGF-β, whereas Smads 1, 5 and 8 are activated by TGF-β-like BMP (Bone morphogenetic proteins). Smads family can be subsequently classified based on their activation by TGF-β or BMP cytokine family. These activated Smads form the complexes with co-Smads, translocate from cytoplasm into nucleus and bind to the distinctive consensus binding sequences on the target promoter region to regulate the transcription of genes. Signosis has developed the Smad2/3 ELISA kit for the analysis of TGF-β/Smad pathway, Smad1/5/8 ELISA kit for the analysis of BMP/Smad pathway, and a combined kit (48 wells for Smad2/3 and 48 wells for Smad1/5/8) to facilitate studying activation of different Smad-related pathways. Signosis基因功能分析，靶标发现和确认，分析开发以及化合物筛选通常需要基于细胞的分析。经过工程改造以通过转基因整合到宿主基因组中表达目的基因的稳定细胞系提供了进行此类分析的有效方法。然而，由于建立这种细胞系的过程繁琐，开发过程耗时，劳动，试剂和材料的昂贵以及单个克隆的转染后验证，是否值得仍然值得商de在单个实验室中开发这种稳定细胞系的方法。  为了促进研究，Signosis现在提供了一系列基因表达或荧光素酶报告基因就绪的稳定细胞系。 萤光素酶报道基因稳定细胞系EGFR稳定表达细胞系HER2（ERBB2）稳定表达的细胞系pLenti-MSCV-EGFR-GFP载体pCMV-EGFR载体pCMV-EGFR-IRES-GFP载体pMSCV-EGFR-GFP载体EGFR突变荧光素酶表达载体GAL4-UAS-luc报告基因稳定细胞系NR LBD驱动的GAL4 Reporter稳定细胞系亲代细胞系