Substrate for cell culture adhesion. Optimal conditions for attachment must be determined for each cell line and application. Recommended concentration for normal human cell attachment is 100-200 µg/cm2.
Gelatin is a heterogeneous mixture of water-soluble proteins from porcine skin. 0.2% Gelatin Solution (GSN) is used to coat the surface of culture vessels to promote cell attachment. It is recommended for the culture of differentiated hESC cells [1, 2], as well as certain primary and immortalized cell types.
Concentration
Sterile 0.2% gelatin in cell culture grade water.
Specifications:
| Catalog No. | 0423 |
| Country of Manufacture | United States |
| Product Code | GSN |
| Size/Quantity | sterile, 100 ml |
| Product Use | THESE PRODUCTS ARE FOR RESEARCH USE ONLY. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro procedures. |
| Storage | Product is stable for 2 years and remains unchanged from the date of receipt when stored at 2 - 8° C. Keep sterile. |
| Shipping Info | Gel ice. |
| References | [1]. Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A. (2000). Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nature Biotechnology. Apr;18(4):399-404. [2]. Ko. J, Kolehmainen, K. , Ahmed, F, Jun, M. B.G, Willerth, S.M. (2012). Towards high throughput tissue engineering: development of chitosan-calcium phosphate scaffolds for engineering bone tissue from embryonic stem cells. Am J Stem Cell;1(1):81-89. |
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