品牌分类
品牌咨询
- 世联博研公司现货供应ROCKLAND IMMUNOCHEMICALS INC.品牌订货号为...
- rockland immunochemicals, inc.|产品目录
- 【ROCKLANDRLB6018】洛克兰(ROCKLAND)夏洛特炫彩背包【行情 报价...
- 【求助】CHEMICON和ROCKLAND公司的中国代理 细胞技术讨论版 ...
- 免疫组化(IHC) | 免疫组化步骤 | 赛默飞世尔官网 | Thermo ...
- 江苏洛克兰经贸发展有限公司
- Akt (PhosphoSer129) Antibody
- antialpha2macroglobulin (human plasma) (goat) antibody...
- Rockland公司实验耗材 Rockland原装|产品目录|代理商|上海玉博生物科...
- 求加精: 中国Rockland公司,哪里有卖?
- ALPHA TUBULIN ANTIBODY EA
- rockland品牌代理 |产品详情
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
AKT3FITCAntibodyPropertiesDescription Anti-AKT3(MOUSE)FluoresceinConjugatedMonoclonalAntibody-200-302-J36 Host Mouse TargetSpecies Human KnownCrossReactivity Human,Mouse,Rat Clonality Monoclonal9A5.H9.G7IgG1kappa Application ELISA:UserOptimized IFMicroscopy:Useroptimized FlowCytometry:UserOptimized WesternBlot:UserOptimized Immunohistochemistry:Useroptimized PhysicalState Lyophilized ShippingCondition WetIce Format IgG1 Label Fluorescein(FITC) FPValue 1-2molesFluorescein(FITC)permoleofIgG1 Concentration 1.0mg/mLbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.5MSodiumChloride,pH7.2 ReconstitutionVolume 50µL ReconstitutionBuffer Restorewithdeionizedwater(orequivalent) Preservative 0.01%(w/v)SodiumAzide StABIlizer 10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree GeneName AKT3 RelevantLinks UniProtKB-Q9Y243 NCBI-NP_001193658.1 GeneID-10000 |
AKT3FITCAntibodyDescription
Background
AKTisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.Anti-AKT3(MOUSE)PEconjugatedMonoclonalAntibodyisidealforinvestigatorsinvolvedinCellSignaling,Cancer,Neuroscience,SignalTransductionresearch.
Synonyms
Mouseanti-AKT3antibodyFITCconjugation,fluoresceinconjugatedMouseanti-AKT3antibody,AKT-3,PKBantibody,PKBgammaantibody,PKBGAMMAantibody,PRKBGantibody,ProteinkinaseAkt3antibody,ProteinkinaseBgammaantibody,RAC-gammaserine/threonine-proteinkinase,RAC-PK-gamma
Immunogen
Anti-AKT3AntibodywasproducedinmicebyrepeatedimmunizationswithasyntheticpeptidecorrespondingtointernalresiduesofhumanAKT3protein.
ImmunogenType
Peptide
StorageCondition
Storevialat4°Cpriortorestoration. Restorewithdeionizedwater(orequivalent).Forextendedstoragealiquotcontentsandfreezeat-20°Corbelow. Avoidcyclesoffreezingandthawing. Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature. Thisproductisstableforseveralweeksat4°Casanundilutedliquid. Diluteonlypriortoimmediateuse. Expirationdateisone(1)yearfromdateofrestoration.
ApplicationNote
Anti-AKT3FITCAntibodyissuitableforFlowCytometry,ELISA,immunohistochemistry,andwesternblotting.Expectabandapproximately56kDainsizecorrespondingtoAKT3proteinbywesternblottingintheappropriatecelllysateorextract.ThismonoclonalantibodyreactswithhumanAKT.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Forimmunohistochemistrywerecommendtheuseoffreshfrozentissues.Attemptsatstainingparaffin-embeddedformalinfixedtissueswerenegative.Nopre-treatmentofsampleisrequired.
Purity/Specifity
Anti-AKT3antibodyisdirectedagainsthumanAKT3.Theantibodydetectsbothunphosphorylatedandphosphorylatedformsoftheprotein.Anti-AKT3antibodywaspurifiedfromascitesbyProteinAchromatography.CrossreactivitywithAKT3fromotherspecieshasnotbeendetermined,however,thesequenceoftheimmunogenshows100%identitytohuman,mouse,andrat,therefore,crossreactivityisexpected.Cross-reactivitywithAKT2andAKThasnotbeendetermined.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.


