Rockland/APC1 phospho S355 Antibody/600-401-856/100 µg

价格
¥7180.00
货号:600-401-856
浏览量:127
品牌:Rockland
服务
全国联保
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商品描述

APC1phosphoS355AntibodyProperties

Description
Anti-APC1pS355(RABBIT)Antibody-600-401-856
Host
Rabbit
TargetSpecies
Human
KnownCrossReactivity
human,mouse,dog,rat,bovine
Clonality
Polyclonal
Application
ELISA:1:10,000-1:35,000
WesternBlot:1:200-1:2,000
ImmunoPrecipitation:1:100
OtherDilution:UserOptimized
PhysicalState
Liquid(sterilefiltered)
ShippingCondition
DryIce
Format
IgG
Label
Unconjugated
Concentration
1.0mg/mLbyUVabsorbanceat280nm
Buffer
0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2
Preservative
0.01%(w/v)SodiumAzide
StABIlizer
None
GeneName
ANAPC1,TSG24
RelevantLinks
NCBI-12056971
UniProtKB-Q9H1A4
GeneID-64682

APC1phosphoS355AntibodyDescription

Background
APC1(alsoknownasAnaphasepromotingcomplexsubunit1,Cyclosomesubunit1,ProteinTsg24,MitoticcheckpointregulatorandANAPC1)is1ofatleast11subunitsoftheanaphase-promotingcomplex(APC),whichfunctionsatthemetaphase-to-anaphasetransitionofthecellcycleandisregulatedbyspindlecheckpointproteins.TheAPCisanE3ubiquitinligasethattargetscellcycleregulatoryproteinsfordegradationbytheproteasome,therebyallowingprogressionthroughthecellcycle.
Synonyms
rabbitanti-APC1pS355Antibody,Anaphase-promotingcomplexsubunit1,APC-1,APC1,Cyclosomesubunit1,Testis-specificgene24protein,Mitoticcheckpointregulator
Immunogen
Thisaffinitypurifiedantibodywaspreparedfromwholerabbitserumproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoaminoacids351-359ofHumanApc1protein.
ImmunogenType
Peptide
PostTranslationalModification
Phosphorylation
StorageCondition
Storevialat-20°Cpriortoopening. Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage. Avoidcyclesoffreezingandthawing. Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature. Thisproductisstableforseveralweeksat4°Casanundilutedliquid. Diluteonlypriortoimmediateuse.
ApplicationNote
ThisaffinitypurifiedantibodyhasbeentestedforuseinELISAandwesternblot.  Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Expectaband~215kDainsizecorrespondingtoAPC1bywesternblottingintheappropriatecelllysateorextract.
Purity/Specifity
Thisproductisanaffinitypurifiedantibodyproducedbyimmunoaffinitychromatographyusingphosphopeptidecoupledtoagarosebeadsfollowedbysolidphaseadsorption(s)againstnon-phosphopeptideandnon-specificpeptidetoremoveanyunwantedreactivities.ThisantibodyisspecificforphosphorylatedhumanAPC1proteinatthepS355residue.ABLASTanalysiswasusedtosuggestreactivitywiththisproteinfromhuman,mouse,dog,rat,andbovinebasedon100%homologyfortheimmunogensequence.CrossreactivitywithAPC1proteinfromchimpanzeeandchickenisexpectedasthesequenceoftheimmunogenonlyvariesbyoneaminoacidinfromthesesources(89%homology).CrossreactivitywithAPC1homologuesfromothersourceshasnotbeendetermined. Minimalreactivityisexpectedwiththenon-phosphorylatedformoftheprotein.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
     美国Rockland是一家老牌抗体原产企业,其位于美国费城郊区的农场保有近20种实验动物,全部按美国农业部实验动物规范饲养和操作,是近20年来美国少数几家成功的大型实验动物商业基地。其产品通过贴牌委托生产,国际代理商和直销等方式行销全球。其中zui的是全线二抗产品,以全面,价格合理为特色。今年,Rockland也加大了在一抗方面的投资力度,新生物分子的抗体数量每年都大幅增长,而这又是以其强大的重组蛋白表达能力为基础的。 美国Rockland公司为生命科学领域提供丰富的、高质量的抗体,包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素(Ubiquitin)等识别抗体,应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。 另外,Rockland也生产特别齐全的二抗,包括纯化、生物素、HRP/ALP、荧光标记二抗,应用十分广泛。   Rockland还对部分设施进行了GMP改造,以吸引大型制药厂的委托订单。同时又以GMP标准为蓝图,提高其他产品的质量标准和管理水平。   Rockland还不断与时俱进,探索其他扩展产品线的渠道。利用周边广布高校和研究机构的便利,Rockland加快和科研单位合作,从深度和广度两维加速开发新产品,正在从一个OEM老厂走到前台,缩短与客户的距离。二抗次级抗体概述二级抗体与一级抗体结合,有助于靶抗原的检测,分选和纯化。为了能够进行检测,第二抗体必须对抗体种类和所使用的一抗的同种型具有特异性,并且通常是共轭的。二次抗体可用于各种类型的测定,包括ELISA或Western Blot,免疫组织化学,流式细胞术。根据一抗(例如,IgG或IgM)的类别,来源宿主以及优选的标记种的种类来选择二抗。大多数一级抗体是IgG类,并且是在一组常见的宿主物种中产生的,包括兔,小鼠,山羊或鸡。因此,通常使用抗小鼠IgG,抗兔IgG,抗山羊IgG或抗鸡多克隆抗体。  选择第二抗体是直接的:选择识别用于产生感兴趣的一级抗体的宿主物种的第二抗体,即选择抗小鼠第二抗体以检测小鼠单克隆一抗。然而,鉴定zui佳的二次抗体需要知识的检测测定。例如:蛋白质印迹和ELISA可以使用比色,化学发光和荧光报告系统进行,而免疫荧光和流式细胞术通常限于荧光报道标记物。在所有这些情况下,需要共轭二抗。 部分二抗信息如下:特色抗体二抗非结合荧光素生物素碱性过氧化物酶磷酸酯酶Anti-HUMAN IgG (H&L) (GOAT) Antibody609-101-123  609-102-123  609-106-123  609-105-123  609-103-123(Min X Bv Ch Gt GP Ham Hs Ms Rb Rt &Sh Serum Proteins)  Anti-MOUSE IgG (H&L) (GOAT) Antibody  610-1102  610-1202  610-1602  610-1502  610-1302  Anti-MOUSE IgG (H&L) (GOAT) Antibody610-101-121  610-102-121610-106-121610-105-121610-103-121(Min X Bv Ch Gt GP Ham Hs Hu Rb Rt &Sh Serum Proteins)  Anti-MONKEY IgG (gamma chain) (GOAT) Antibody617-101-012617-102-012617-106-012617-105-012617-103-012Anti-RABBIT IgG (H&L) (GOAT) Antibody  611-1102  611-1202  611-1602  611-1502  611-1302  Anti-RABBIT IgG (H&L) (GOAT) Antibody611-101-122611-102-122611-106-122611-105-122  611-103-122  (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt &Sh Serum Proteins)  Anti-SHEEP IgG (H&L) (RABBIT) Antibody613-4102  613-4202  613-4602613-4502  613-4302F(ab')2 Anti-HUMAN IgM Fc5µ (GOAT) Antibody709-1131  709-1231709-1631709-1531  709-1331