Description:

flashBACGOLD(cat#100200/100201/100202/100203) providessuperiorlevelsofexpressionforanyproteinthatisdestinedforsecretionortobeinsertedinthemembrane,orforanyproteinthatmightbeparticularlyliabletodegradation.flashBACGOLDisabaculovirusexpressionvectorthathasbeendesignedtoreduceproteolysis,maximiseproteinsecretionandimprovemembraneproteintargetingandisbasedonourpatentedflashBAC™system.ThedeletionofbothchiAandv-cathgenesfromflashBACGOLDhasimprovedtheefficacyofthesecretorypathwayandresultedinagreatlyenhancedyieldofrecombinantproteinsthataresecretedormembranetargeted(incomparisontorecombinantvirusesthatencodechiAandv-cath).

UsingtheflashBAC™systemiseasierandquickerthanotherbaculovirusexpressionsystemsbecausethereisnoneedtoseparaterecombinantvirusfromparentalvirusbyplaque-purificationoranyothermeans;onlyrecombinantvirusisproducedaftertheco-transfection. Becausetheproductionofrecombinantvirushasbeenreducedtoaonestepprocedureininsectcells,itamenabletohighthroughputandautomatedproductionsystems. However,itisalsoofbenefittothesmallresearchgroupjustrequiringoneorafewrecombinantbaculovirusespreparedinindividualdishesofcells.

Baculovirusgenomescontainseveralauxillarygenes,whicharenon-essentialforreplicationininsectcellculture.Oneoftheseischitinase(chiA),whichencodesanenzymewithexo-andendochitinaseactivity.Inaninfectedinsect,chitinasetogetherwiththecysteineproteasecathepsin,facilitateshostcuticlebreakdownandtissueliquefactionattheverylatestagesofinfection,soreleasingthevirustoinfectmorehosts.ConfocalandelectronmicroscopyobservationsofinsectcellsinfectedwithAcMNPVhaveshownthatchitinaseistargetedtotheendoplasmicreticulumwhereitisdenselypackedinaparacrystallinearray,severelycompromisingthefunctionandefficacyofthesecretorypathway.DeletionofchiAfromflashBAC™hasimprovedtheefficacyofthesecretorypathwayandresultedinagreatlyenhancedyieldofsecretedandmembranetargetedproteins. Deletionofv-cathreducesthechancesoftherecombinantproteinbeingdegraded.

Oxford Expression Technologies是杆状病毒蛋白表达系统的专家。我们所有的产品都经过优化，以确保快速，直接的重组病毒生产和蛋白质表达。我们的在线商店提供了您可能需要的一切，从革命性的 Flash BAC™杆状病毒表达载体和杆状病毒qPCR滴定试剂盒，到转染试剂和转移质粒。