Description:

pOET2(cat#200103)(10ug)isabaculovirustransfervectordesignedforhighlevelexpressionofforeigngenesunderthepowerfulAcMNPVpolyhedrin(polh)promoter. Thevectorissmallerthanotheravailabletransfervectors(4547bp)whichgreatlyfacilitatesthecloningsteps.IthasabacterialoriginofreplicationandanampicillinresistancegeneforselectioninE.coli.Thepolhsequenceshavebeenreplacedbyamultiplecloningsite(MCS)inthereverseorientationtopOET1,containinguniquerestrictionsitesforinsertionoftheforeigngene.TheAcMNPVsequencesflankingthegeneinthetransfervector’sMCSallowrecombinationwiththeviralDNAtoinserttheexpressioncassetteintothepolhlocus.pOET2iscompatIBLewithanybaculovirussystemthatutilizeshomologousrecombinationininsectcells.

Variations

pOET1 hasthesameMCSaspOET2butinthereverseorientation.

 

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