Description:

(cat#100400/100401)

flashBAC™istheeasiestandthequickestbaculovirusproteinexpressionsystemavailable.TheflashBAC™expressionvectorsystemisanewplatformtechnologyfortheproductionofrecombinantbaculoviruses,geneticallyoptimizedforproducing100%recombinantvirusinasinglestepthuseliminatingtheneedforplaquepurificationorotherscreeningsystems.Thegeneticoptimizationnotonlypreventparentalvirusfromreplicating,butalsosignificantlyincreasesproteinyield.TheflashBAC™expressionsystemhasbeenspecificallydevelopedforoptimalproteinproductionininsectcells.TheflashBAC™DNAisabaculovirusgenomethathasbeengeneticallyoptimisedtofunctionasarecombinantproteinexpressionvector.Thesystemoffershighlevelsofproteinexpression,iseasytouseandisalsobackcompatIBLewithalltransfervectorsbasedonhomologousrecombination.InthiswaytheflashBAC™systemhasbecometheexpressionofchoiceforthoseneedingoptimalproteinexpressionininsectcells,andincreasinglyinmammaliancellsviatheBacMamsystem.

ThereareaseriesofflashBAC™expressionvectors,eachvaryinginthenumberofgenesdeletedfromthebaculovirusgenometosuitexpressionofdifferentproteins. TheseSelectionBoxesenableyoutotryoutdifferentversionsofflashBAC™inoneconvenientkit:SelectionBoxescontaineitherasingle3reactionkitof flashBAC™,flashBACGOLDandflashBACULTRA(Box1)orasingle3reactionkitof  flashBAC,flashBACGOLD,flashBACULTRAandflashBACPRIME(Box2).TheoriginalflashBACexpressionvectorisagoodchoiceforprojectswherethegenetobeexpressedislikelytobesimpleandexpressedinthecytoplasmornucleus.flashBACGOLDisenhancedbythedeletionofthechitinaseandcathepsinproteasegenes.Itprovidessuperiorlevelsofexpressionforanyproteinthatisdestinedforsecretionortobeinsertedinthemembrane,orforanyproteinthatmightbeparticularlyliabletodegradation.flashBACULTRAcontainsdeletionsofthechitinaseandcathepsingenes,asabove,butalsodeletionsofthep10,p74andp26genesandprovidesthebestchoiceforthemostdifficulttoexpressproteins;uitableforcytoplasmic,nucleus,secretedandmembraneproteins.flashBACPRIMEcontainsnogenedeletionsandhasprovenusefulforthereleaseandsubsequentpurificationofintracellularVLPsandproteincomplexes. Ifitisastarterkityouareafter,baculoCOMPLETEistheperfectchoice.Thiskitcontainsallthecomponentsneededtogetstartedwithrecombinantproteinexpressionininsectcells.

Oxford Expression Technologies是杆状病毒蛋白表达系统的专家。我们所有的产品都经过优化，以确保快速，直接的重组病毒生产和蛋白质表达。我们的在线商店提供了您可能需要的一切，从革命性的 Flash BAC™杆状病毒表达载体和杆状病毒qPCR滴定试剂盒，到转染试剂和转移质粒。