Lucigen/EZ-Tn5和贸易&书信电报;T7/KAN-2>;启动子插入试剂盒/EZI03T7/10反应

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¥10800.00
货号:EZI03T7
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品牌:Lucigen
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商品描述

RandominsertionofT7promotertodrivegeneexpressionwithT7RNApolymerase

  • InsertT7promoterrandomlyintoanyDNAsequenceinvitroFormerly from Epicentre
  • IsolatepositivecloneswiththeselectablekanamycinMarker
  • Expressresultingproductsinvivoorinvitro
  • MinimizeinsertionbiaswiththehyperactiveTn5system,knownforhighestlevelofrandomness

Applications

  • RandominsertionofasingleT7RNAPolymerasepromotertosynthesizeRNAfromanyregionofclonedDNA.

TheEZ-Tn5™<T7/KAN-2>PromoterInsertionKit*providesaneasyandreliablemethodtorandomlyinsertatransposoncontainingaphageT7RNApolymerasepromoterintoanyDNAmoleculeinvitro.Thetransposonendhasnoterminationsequences,soRNAcanbeproducedfromchoseninsertionclonesbyinvitrotranscriptionfromtheT7RNApolymerasepromoterusing,forexample,anyofEpicentre'sT7RNAPolymeraseTranscriptionKits.RNAcanalsobegeneratedforinvivoexpressionstudiesincellshavinganinducIBLeT7RNApolymerasegene.


Figure1(clicktoenlarge).RNAtranscriptscanbeproducedfrominsertionclonesinvitroorinvivousingtheEZ-Tn5™<T7/KAN-2>PromoterInsertionKit.

*Coveredbyissuedand/orpendingpatents,exclusivelylicensedorassignedtoEpicentre®(anIllumina®Company).

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Contents:EZ-Tn5™Transposase,EZ-Tn5™Transposon,EZ-Tn5™10XReactionBuffer,EZ-Tn5™10XStopSolution,ForwardandReversePrimers,ControlTargetDNA,SterileWater 美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。