TheMasterPure™GramPositiveDNAPurificationKitprovidesallofthereagentsneededtopurifyDNAfromGrampositivebacteria.ThesebacterialysemorereADIlyaftertreatmentwithReady-Lyse™LysozymeandtheGram-PositiveCellLysisSolutionincludedinthekit.Ready-LyseLysozymeisastablesolutionofarecombinantlysozymefromanonmammalian,nonaviansource.IthashighspecificactivityanddoesnotbindDNA.^1,2

ExamplesofGram-positivebacteriatestedwiththiskitarelistedinTable1.ThesizerangeofDNApurifiedbytheMasterPureGramPositiveDNAPurificationKitisshowninFig.1.TheDNApurifiedusingthekitissuitableforPCRanalysis(Fig.2),andothermolecularbiologyapplications.

Species	CultureMedium	Ready-Lyse™Incubation	DNAYieldµg/ml
Bacillussubtilis	Brain-HeartInfusion(BHI)	30min	9.0
Listeria monocytogenes	BHI	Overnight	3.3
Staphylococcusaureus	BHI	Notneeded	8.0
Streptococcusmutans	Todd-Hewitt	Overnight	3.0
Lactococcuslactis	M17	30min	1.1

Table1.IncubationtimesneededforDNArecoveryfrom1mlofGrampositivebacterialculture.

---

	.
Figure1.ElectrophoreticanalysisofDNApurifiedusingtheMasterPure™GramPositiveDNAPurificationKit.PanelA:DNApurifiedfromB.subtilis(ATCC6051)wasseparatedona1%agarosegelandstainedwithSYBR®Gold.LaneM,kilobaseladder;lane1,300ngofB.subtilisDNA.PanelB:Pulse-fieldgelelectrophoresisofB.subtilisDNA.LaneM,PhagelamBDaladder;lane1,300ngofDNA;lane2,600ngofDNA	Figure2.PCRanalysisofDNApurifiedusingtheMasterPure™GramPositiveDNAPurificationKit.B.subtilisDNAwasamplifiedbyPCRusingBacF/BacRprimers,andtheproductswereanalyzedbyagarosegelelectrophoresis.LaneM,100-bpladder;lane1,PCRproductfrom0.5ngofB.subtilisDNA;lane2,no-templatecontrol.

---

References

1. Hoffman, L. and Jarvis, B. (2003) Epicentre Forum 10(3), 3.
2. Jarvis,B.andHoffman,L.(2004)EpicentreForum11(3),7.
3. Luna,V.A.etal.(2006)J.Clin.Microbiol.44,2367.
4. Li,J.etal.(2007)Immunol.75,1811.

ORDERINFORMATION

Contents:Gram-PositiveCellLysisSolution,MPCProteinPrecipitationReagent,Ready-Lyse™Lysozyme,ProteinaseK,TEBuffer,RNaseA. 美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。