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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
RemoveunwantedRNAbydigestingwiththisheatlABIle,non-sequencespecificribonuclease
Complete:UnlikeRNaseA,RNaseIdoesnothaveabasepreferenceanddigestsbetweenalldinucleotidepairsinsingle-strandedRNA- HeatLabile:RNaseIiseasilyinactivatedbyheating70°Cfor15minutes
RNaseIdegradessingle-strandedRNAtonucleoside3´monophosphatesvia2´,3´cyclicmonophosphateintermediatesbycleavingbetweenalldinucleotidepairs,2,3unlikeRNaseA,whichcleavesonlyaftercytosineanduridine.Inaddition,theenzymeiscompletelyinactivatedbyheatingat70°Cfor15minutes,eliminatingtherequirementtoremovetheenzymepriortomanysubsequentprocedures.
Applications
- RemovalofRNAfromDNApreparations.1
- RNaseprotectionassaystodetectsingle-basepairmismatchesinRNA:RNAandRNA:DNAhybrids.1
UnitDefinition:OneunitofRNaseIdegrades100ngofE.coliribosomalRNApersecondintoacid-solublenucleotidesat37°Cunderstandardassayconditions.
DilutionandStorageBuffer:50%glycerolcontaining50mMTris-HCl(pH7.5),100mMNaCl,and0.1mMEDTA.
QualityControl:RNaseIisfreeofdetectableexo-andendodeoxyribonucleaseactivities.
References
- Johnson,M.(1996)EpicentreForum3(4),7.
- Shen,V.andSchlessinger,D.(1982)TheEnzymesXV(PartB),501.
- delCardayré,S.B.andRaines,R.T.(1995)Anal.Biochem.225,176.
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| Figure1.RemovalofRNAfromplasmidDNApreparations.PlasmidDNAwaspreparedfrom1.5mLofanovernightculture,sUSPendedin50µLTEbuffer(10mMTris-HCl(pH8),1mMEDTA),andtreatedwithRNaseA,RNaseI,ornoenzyme.FiveµLofeachreactionwereresolvedbyagarosegelelectrophoresisandvisualizedbystainingwithethidiumbromide.Lane1,noRNase;Lane2,1.5UofRNaseI;Lane3,20µg/mLRNaseA;MW,1-kbladder.ArrowdenotessmallundigestedRNA. |
