T4DNALigasecatalyzestheformationofaphosphodiesterbondbetweentheterminal5′phosphateand3′hydroxylgroupsofduplexDNAorRNA.TheenzymeefficientlyjoinsbluntandcohesiveendsandrepairssinglestrandednicksinduplexDNA,RNAorDNA/RNAhybrids.

T4DNALigaseVersion	Sizes	LigaseConcentration	ProvidedBuffer
LowConcentrationLigase	1,500U 7,500U	2U/µL	10XT4DNALigaseBuffer
HighConcentrationRapidKit	1,500U 7,500U	10U/µL	2XRapidLigationBuffer, 10XT4DNALigaseBuffer

10XT4DNALigaseBufferiscomposedof500mMTris-HCI,100mMMgCl₂,50mMdithiothreitol,10mMATP,pH7.6@25°C.

2XRapidLigationBufferiscomposedof132mMTris-HCI,20mMMgCl₂,2mMdithiothreitol,2mMATP,15%PEG,pH7.6@25°C.

 

 

UnitDefinition:OneWeissUnitisdefinedastheamountofenzymerequiredtoconvert1nmolof³²P-labeledinorganicpyrophosphateintoNoritadsorbablematerialin20minutesat37°C,usingspecifiedreactionconditions.Note:1WeissUnitisapproximately67cohesiveendunits.

Source:ArecombinantE.colistraincarryingtheclonedT4DNALigasegene.

ORDERINFORMATION

Storagebuffer:T4DNALigaseissuppliedin10mMTris-HCl,50mMKCl,1mMdithiothreitol,0.1mMEDTA,0.1%TritonX-100,50%glycerol,pH7.5@25°C.

美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。