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Clonerepetitivesequencesandlentivirallibraries
- StABIlizedirectrepeatsandcreatelentiviralconstructs
- GeneratelentiviralguideRNAlibraries
- RecommendedinCRISPRGeCKOlibraryprotocols
- Chooseelectrocompetentorchemicallycompetentcells
- Highestefficiencycommerciallyavailablecellsforlentiviralcloning: over1×107cfu/µg(chem)or1×1010cfu/µg(electro)
TransformationofEnduraElectrocompetentCellswithGeCKOlentivirallibrariesmayrequireextraRecoveryMedium. OrderextraRecoveryMediumbelow.
FreeSampleAvailable
Conserveclonesthatcontainunstablesequences!
MaintainunstableDNA.Lucigen’sEnduraCompetentCellsareacommonlyusedstrainforcloningsequencesthatsufferunwantedrecombinationeventsinotherstrains.Cloneswithinvertedrepeatsorothersequencespronetorecombinationarecommonlyfoundinretroviralgenes,andrequirecellssuchasEnduratobepropagatedstably.
Excellentvalueandefficiency!SwitchtoEnduracellstodayandexperienceahigherlevelofefficiencyandasavingsinyourcloningbudget.
ChemicalorElectrocompetent.Whichevermethodoftransformationyouprefer,Lucigencangiveyoubetterefficiencyand/orbetterprices.
Figure1.Costperreactionofchemicallycompetentcellsofclone-stabilizingstrainsfromothersuppliers.
Figure2.Transformationefficienciesofelectrocompetentclone-stabilizingstrains.
ORDERINFORMATION
Endura™CompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedas SOLOs (1transformationpertube)orDUOs (2transformationspertube)asindicated.EnduraElectrocompetentcellsareprovidedinDUOsformatonly.RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.
PLEASENOTE: Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells. PleasecontactLucigen.
NOTE: TransformationofEnduraElectrocompetentCellswithGeCKOlentivirallibrariesmayrequireextraRecoveryMedium. TheZhanglabprotocol(availablefromAddgene)recommends~2mLofRecoveryMediumpertransformation. LucigenEndurakitsprovide1mLofRecoveryMediumpertransformation. OrderextraRecoveryMediumaboveorvisittheRecoveryMediumproductpagehere!
美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。
