Lucigen/DNATerminator®;端部修理包/40035-2/50 rxns

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¥6540.00
货号:40035-2
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品牌:Lucigen
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商品描述
  • Repairshearedorrestriction-digestedDNAends
  • Increasecloningefficiencyasmuchasa500%
  • Optimizedsystemforconsistent&reliableresults

RepairDNAends

Double-strandedDNAfragmentsforlibraryconstructionorlarge-scalesequencingareoftengeneratedbymechanicallyshearinglarger(genomic)DNA,aprocessthatprimarilyleavesunevenends.LargeDNAmayalsobecutwithrestrictionenzymestogeneratesmallerfragmentsforsubcloning.ShearedorrestrictedDNAfragmentsmustbe“end-repaired”beforeligationintoblunt-endcloningvectors(e.g.,Lucigen’sCloneSmart®,CopyRight®,orBigEasy®systems).

TheDNATerminatorEndRepairKitcreatesblunt,5'phosphorylatedendsonanytypeofshearedorrestriction-digestedDNAfragment,ensuringthehighestpossIBLecloningefficiency(Figure1).

DNA Terminator Kit

Figure1.EffectofDNAshearingandend-repairmethodsonlibraryconstructionefficiency.Shotgunlibrarieswereconstructedusing250ngoflamBDaDNAshearedbysonication(Son)orbytheGeneMachines®HydroShear™device(HS).ShearedDNAwasrepairedwithT4DNApolymeraseandKlenowfragment(TK)orDNATerminatorEndRepairKit(DT)andclonedusingLucigen’sCloneSmart®system.Valuesarecolonyformingunits(cfu)perlibrary.

ORDERINFORMATION

TheDNATerminatorEndRepairKitcontains:DNATerminatorEndRepairEnzymeMix,EndRepairBuffer,andacompleteprotocol. 美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。