Description:

pOET2N/C_6xHisisabaculovirustransfervectordesignedforhighlevelexpressionofforeigngenesunderthepowerfulAcMNPVpolyhedron(polh)promoter.ThevectorencodesanNterminal6×His-Tag®fusionsequencethatmaybeutilizediftheinsertincludesastopcodon.Thisgreatlyeasesthepurificationoftherecombinantproteinsincethe6×His-containingfusionproteinsbindwithhighaffinitytoNi-NTAAgarose.Ifrequired,the6×His-Tag®canberemovedbyincubatingthefusionproteininthepresenceoftheproteinasecleavageenzymeThrombin.Thereisalsoa6×His-Tag®forC-terminalfusionswheretheinsertsownstartcodoncanbeusedtoreplacethestartcodonsuppliedinpOET2N/C_6xHis.pOET2N/C_6xHisissmallerthanotheravailabletransfervectors(4626bp)whichgreatlyfacilitatethecloningsteps.IthasaColE1originofreplicationandanampicillinresistancegeneforselectioninE.coli.Thepolhsequenceshavebeenreplacedbyamultiplecloningsite(MCS)containinguniquerestrictionsitesforinsertionoftheforeigngeneinthecorrectorientation,asshownonthecircularmap.ThecodingstrandoftheMCSastranscribedfromthepolhpromoterisshownbelowthecircularmap.ThePacIsiteattheendoftheMCSprovidestranslationalstopcodonsinallthreereADIngframesforexpressionoftruncatedproteins.TheAcMNPVsequencesflankingthegeneinthetransfervectorsMCSallowrecombinationwiththeviralDNAtoinserttheexpressioncassetteintothepolhlocus.pOET2N/C_6xHisiscompatIBLewithanybaculovirussystemthatutilizeshomologousrecombinationininsectcells.

AdditionalInformation:

GenWay公司是为了探索和开发人类蛋白组而成立的。公司的总任务就是用基因特异性抗体來筛选和鉴定新蛋白、其功能及其编码基因，旨在成为以抗体介导的蛋白组研发的领域先锋。基因纬公司已成功地发展出一套有自己知识产权的、新穎、高效、低耗、高筛选通量的鉴定方法――抗体介导的蛋白和基因鉴定。公司提供大量的高质量的蛋白和相应的鸡抗体产品，是蛋白质组学研究的重要工具和有力助手。GenWay Biotech的主要产品包括：ProteinsAntibodiesELISA KitsSecondary AntibodiesReagentsPeptidesMolecular Biology ProductsActive ProteinsEnzymatic AssaysPCR Kits

Name	pOET2N/C_6xHis™TransferVector
Concentration	LotSpecific
StABIlity	1year
Storage	-20C
IntendedUse	ResearchUseOnly