Description:

Description:ThePDE5AAssayKitisdesignedforidentificationofinhibitorsofPDE5Ausingfluorescencepolarization.Phosphodiesterases(PDEs)playanimportantroleindynamicregulationofcAMPandcGMPsignaling.PDE5A,alsoknownascGMP-inhibitedphosphodiesterase,hasbeenimplicatedincardiovascularfunctionandfertility.TheassayisbasedonthebindingofafluorescentnucleotidemonophosphategeneratedbyPDE5Atothebindingagent.ThePDE5Ainhibitorscreeningassaykitcomesinaconvenient96-wellformat,withpurifiedPDE5Aenzyme,fluorescentlylabeledPDE5Asubstrate(cGMP),bindingagent,andPDEassaybufferfor100enzymereactions.ThekeytothePDE5AAssayKitisthespecificbindingagent.Usingthiskit,onlytwosimplestepsonamicrotiterplatearerequiredforPDE5Areactions.First,thefluorescentlylabeledcGMPisincubatedwithasamplecontainingPDE5Afor1hour.Second,abindingagentisaddedtothereactionmixtoproduceachangeinfluorescentpolarizationthatcanthenbemeasuredusingafluorescencereader.COMPONENTS:PDEassaybuffer:6ml;FAM-Cyclic-3?,5"-AMP:50µl@20µM;Bindingagent:100µl;Bindingagentdiluent:10ul;1blackplate,1ugPDE5A.APPLICATIONS:GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.STORAGE:-20°Cor-70°C.REFERENCES:1.MauriceDH.Front.Biosci.2005;10:1221-8.

Format:assaykit

ScientificCategory:PDE

AdditionalInformation:

GenWay公司是为了探索和开发人类蛋白组而成立的。公司的总任务就是用基因特异性抗体來筛选和鉴定新蛋白、其功能及其编码基因，旨在成为以抗体介导的蛋白组研发的领域先锋。基因纬公司已成功地发展出一套有自己知识产权的、新穎、高效、低耗、高筛选通量的鉴定方法――抗体介导的蛋白和基因鉴定。公司提供大量的高质量的蛋白和相应的鸡抗体产品，是蛋白质组学研究的重要工具和有力助手。GenWay Biotech的主要产品包括：ProteinsAntibodiesELISA KitsSecondary AntibodiesReagentsPeptidesMolecular Biology ProductsActive ProteinsEnzymatic AssaysPCR Kits

Name	PDE5AAssayKit
RelatedProductNames	PDE,phosphodiesterase,screening,inhibitors,PDE5
Format	AssayKit
Storage	Mixedstorage
IntendedUse	ResearchUseOnly