Description:

Description:ThePDE1BAssayKitisdesignedforidentificationofPDE1Binhibitorsusingfluorescencepolarization.TheassayisbasedonthebindingofafluorescentnucleotidemonophosphategeneratedbyPDE1Btothebindingagent.PDE1Bisacalcium-dependentcyclicnucleotidephosphodiesterasethatishighlyexpressedinthestriatum.Itplaysthephysiologicalroleinthecentralnervoussystem.ThePDE1Binhibitorscreeningassaykitcomesinaconvenient96-wellformat,withpurifiedPDE1Benzyme,fluorescentlylabeledPDE1Bsubstrate(cAMP),bindingagent,andPDEassaybufferfor100enzymereactions.ThekeytothePDE1BAssayKitisthespecificbindingagent.Usingthiskit,onlytwosimplestepsonamicrotiterplatearerequiredforPDE1Breactions.First,thefluorescentlylabeledcAMPisincubatedwithasamplecontainingPDE1Bfor1h.Second,bindingagentisaddedtothereactionmixtoproduceachangeinfluorescentpolarizationthatcanthenbemeasuredusingafluorescencereader.COMPONENTS:PDEassaybuffer:6mlFAM-Cyclic-3?,5"-AMP:50µl@20µMBindingagent:100µlBindingagentdiluent:10ul1blackplate200ngPDE1Bfor100reactionsAPPLICATIONS:GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.STORAGE:-20°Cor-70°C.REFERENCES:1.SiuciakJA,McCarthySA,ChapinDS,ReedTM,VorheesCV,RepaskeDR.Neuropharmacology2007;53(1):113-24.

Format:assaykit

ScientificCategory:PDE

AdditionalInformation:

GenWay公司是为了探索和开发人类蛋白组而成立的。公司的总任务就是用基因特异性抗体來筛选和鉴定新蛋白、其功能及其编码基因，旨在成为以抗体介导的蛋白组研发的领域先锋。基因纬公司已成功地发展出一套有自己知识产权的、新穎、高效、低耗、高筛选通量的鉴定方法――抗体介导的蛋白和基因鉴定。公司提供大量的高质量的蛋白和相应的鸡抗体产品，是蛋白质组学研究的重要工具和有力助手。GenWay Biotech的主要产品包括：ProteinsAntibodiesELISA KitsSecondary AntibodiesReagentsPeptidesMolecular Biology ProductsActive ProteinsEnzymatic AssaysPCR Kits

Name	PDE1BAssayKit
RelatedProductNames	PDE,phosphodiesterase,screening,inhibitors,PDE1B
Format	AssayKit
Storage	Mixedstorage
IntendedUse	ResearchUseOnly