Additional Information:

Name	Canine IgA ELISA
Related Product Names	IgA, Immunoglobulin A, Dog IgA, Canine Immunoglobulin A, Dog Immunoglobulin A ELISA
Alias Symbols	IGHA1, IGHA2, IgA, Ig alpha 1 chain C region, Ig alpha 2 chain C region
Protein Name	IgA
Species Reactivity	Dog
Assay Info	Assay Methodology: Quantitative Sandwich ELISA
Sensitivity	7.767 ng/ml
Kit Range	31.25 ng/ml - 1,000 ng/ml
Homology	Dog
Standard Range	1000 ng/ml-15.625 ng/ml
Sample Type	Serum, plasma and other biological fluids
Product Format	In Solution
Specimen/Volumes	Refer To Manual
Concentration	Lot Specific
Applications	ELISA
Reconstitution and Storage	1. DILUENT The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C. 2. WASH SOLUTION The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C. 3. ENZYME-ANTIBODY CONJUGATE Undiluted horseradish peroxidase anti-IgA conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours. 4. CHROMOGEN-SUBSTRATE SOLUTION The Substrate Solution should be stored at 4-8C and is stable until the expiration date. 5. STOP SOLUTION The Stop Solution should be stored at 4-8C and is stable until the expiration date. 6. ANTI-DOG IgA ELISA MICRO PLATE Anti-Dog IgA coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack. 7. DOG IgA CALIBRATOR Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity	Canine
Kit Size	96 well
Kit Principle	Genway Biotech IgA ELISA Kit (Dog) (GWB-374Z20) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for IgA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-IgA - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IgA captured in the well.
Kit Component	Component Amount Anti-Dog IgA Microplate 96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate 50 mL 20X Wash Solution 50 mL 100X Enzyme-Antibody Conjugate 150 uL Chromogen-Substrate Solution 12 mL Stop Solution 12 mL Dog IgA Calibrator 1
Kit Duration	70 Minutes
Storage	Refer to kit instructions for component storage
Lead Time	Domestic: within 1 week delivery International: 1 week
Intended Use	Research Use Only
Additional Information	Intended Use: The total Dog IgA test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgA in biological samples of Dogs.
::	Reagents: 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ul of affinity purified anti-Dog IgA antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-DOG IgA ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Dog IgA. 7. DOG IgA CALIBRATOR One vial containing Dog IgA calibrator.

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