Description:

1.IntendedUse TheGenWayMycoplasmapneumoniaeIgAantibodyELISAkithasbeendesignedforthe detectionandthequantitativedeterminationofspecificIgAantibodiesagainstMycoplasma pneumoniaeinserumandplasma.FurtherapplicationsinotherbodyfluidsarepossIBLeandcanbe requestedfromtheTechnicalServiceofGenWay. Thisassayisintendedforresearchuseonly. 

2.GeneralInformation MycoplasmsbelongtotheMollicutesclass.Commoncharacteristicsofthesixeubacterialgenerais thelackofabacterialcellwall,osmoticfragilityandsmalldimensions,whichallowapenetration througha0.45µmfilter.Alsothegenomewith 600kbpissignificantlysmallercomparedwith gram-positiveandgram-negativebacteria.Outofthisreasontheyhaveneverbeenfoundasfreely livingorganisms.InnatureMollicutesdependonahostcell,respectively,onahostorganismlikea parasite. Mycoplasmapneumoniaeisahumanpathogenicbacteriumcausingtracheobronchitisandprimary atypicalpneumonia.Associatedwiththehostcell,surfacecolonizationofhumanrespiratorytract epithelialcellstakesplace.Alsosecondary diseaseslikeinfarction,encephalitis,chronic neuropathyandtheGuillain-BarresyndromecaninsomecasesbeconnectedwithaM.pneumoniae infection. Inthelaboratory,M.pneumoniaecanbegrownwithoutahostcellinrichmediumsupplemented with10-20%horseserum.BesidesthecoldagglutinintestandcomplementfixationreactionCF, ELISAisthemethodofchoice,whichshowsanexcellentsensitivityandthepossibilityto differentiatebetweentheimmunogloblinclasses. SpecificIgAantibodiesweredevelopedmoreregularlyandmorerapidlythanIgMduringanacute infection.IgAtitresalsostartedtodecreaseearlierthanIgMorthelate-peakingIgGresponse. Itcouldbeshowninvariousstudies,thatthedeterminationofallthethreeimmunoglobulinclasses isnecessary,tomonitoreachstepofthecourse.

3.PrincipleoftheTest TheGenWayMycoplasmapneumoniaeIgAantibodytestkitisbasedontheprincipleofthe enzymeimmunoassay(EIA).Mycoplasmapneumoniaeantigenisboundonthesurfaceofthe microtiterstrips.Dilutedsampleserumorready-to-usestandardsarePipettedintothewellsofthe microtiterplate.AbindingbetweentheIgAantibodiesoftheserumandtheimmobilized Mycoplasmapneumoniaeantigentakesplace.Afteraonehourincubationatroomtemperature,the plateisrinsedwithdilutedwashsolution,inordertoremoveunboundmaterial.Thenready-to-use anti-human-IgAperoxidaseconjugateisadded andincubatedfor30minutes.Afterafurther washingstep,thesubstrate(TMB)solutionispipettedandincubatedfor20minutes,inducingthe developmentofabluedyeinthewells.Thecolordevelopmentisterminatedbytheadditionofa stopsolution,whichchangesthecolorfrombluetoyellow.Theresultingdyeismeasuredspectrophotometricallyatthewavelengthof450nm.The concentrationofIgAantibodiesisdirectly proportionaltotheintensityofthecolor. 

AdditionalInformation:

GenWay公司是为了探索和开发人类蛋白组而成立的。公司的总任务就是用基因特异性抗体來筛选和鉴定新蛋白、其功能及其编码基因，旨在成为以抗体介导的蛋白组研发的领域先锋。基因纬公司已成功地发展出一套有自己知识产权的、新穎、高效、低耗、高筛选通量的鉴定方法――抗体介导的蛋白和基因鉴定。公司提供大量的高质量的蛋白和相应的鸡抗体产品，是蛋白质组学研究的重要工具和有力助手。GenWay Biotech的主要产品包括：ProteinsAntibodiesELISA KitsSecondary AntibodiesReagentsPeptidesMolecular Biology ProductsActive ProteinsEnzymatic AssaysPCR Kits

Name	MycoplasmapneumoniaeIgAELISA
RelatedProductNames	MycoplasmapneumoniaeIgAELISA
IntendedUse	ResearchUseOnly