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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Overview |
|
Product |
StripOBufferTM |
Description | StripOBufferTMisaspeciallyformulatedwaterbasedbufferthatwillstripantibodiesfromantigensin5-10minutesatroomtemperature |
Volume | 100ML |
Concentration | 10Xsolution |
Storage | Roomtemperatureforshort-termstorage |
Applications | 25mLofStripObuffercanbeusedon25-50mini-blots. |
Advantages |
|
- Savetimebyre-usingblots
- Preserveproteinandantigensamples
- EliminateissueswithproperequalproteinloADIng
- Comparephosphorylatedandnon-phosphorylatedstateofthesameprotein
onthesameblot - Reconfirmproteinexpressionbyre-probingthesameblotwithdifferentsetof
antibodiesraisedagainstantibodiesfromdifferentvendorsorsameordifferentepitopes - Preformmultiplestripping’swhilemaintainingantigenicity
- Noheatingrequired
- Noβ-mercaptoethanolrequired–Nosmell
- CompatIBLewithNitrocelluloseandPVDFmembranes
Protocol |
|
- Dilute(1:10)thesuppliedbufferindistilledwaterpriortouse.
- Rinsethemembraneindistilledwaterfirstandtheninstrippingbufferatleastonce.
Multiplerinseswithbufferresultsinclearerbandsonautoradiograms - Immersethemembranecompletelyin10mLofstrippingbufferforeach
mini-gelmembrane. - Gentlyshakethemembraneonarockerfor5-10minutesatroomtemperature.
- Rinsethemembranewithdistilledwater.
- RinsethemembranewithTBSTorthebufferinwhichyourprimaryantibodyisdiluted.
- Proceedforblockingstage.


