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Greenfluorescentprotein(GFP),a27kDaproteinderivedfromthejellyfishAequoreavictoria,emitsgreenlight(emissionpeak509nm)whenexcitedbybluelight(excitationpeak395nm). GFPhasbecomeaninvaluabletoolincellBIOLOGyresearch,sinceitsintrinsicfluorescencecanbevisualizedinlivingcells.GFPfluorescenceisstableunderfixationconditionsandsuitableforavarietyofapplications.GFPhasbeenwidelyusedasareporterforgeneexpression,enablingresearcherstovisualizeandlocalizeGFP-taggedproteinswithinlivingcellswithouttheneedforchemicalstaining.OtherapplicationsofGFPincludeassessmentofprotein-proteininteractionsthroughtheyeasttwohybridsystemandmeasurementofdistancebetweenproteinsthroughfluorescenceenergytransfer(FRET)protocols.GFPisusedtomeasuresinglecellmetastasisandsuccessfulproliferationofstemcells.
