The DetectX^® Retinol Binding Protein (RBP) Multi-Format ELISA Kit is designed to allow for the measurement of both high and low RBP levels in a variety of samples including dried blood spots, serum, EDTA and heparin plasma and urine with the same kit. The kit uses native human RBP as a standard. Standards or diluted samples are added to a coated microtiter plate along with a RBP-peroxidase conjugate and the binding reaction initiated by addition of a RBP-specific sheep antibody. After a 60-minute incubation, the plate is washed and TMB substrate solution added. After 30 minutes, the color development is stopped and the intensity of the generated signal is read at 450nm.

Retinol binding protein (RBP) is from a family of structurally related proteins that bind small hydrophobic molecules such as bile pigments, steroids, odorants, etc. RBP is a 21 kDa highly conserved, single-chain glycoprotein, consisting of 182 amino acids with 3 disulfide bonds, that has a hydrophobic pocket which binds retinol. In urine RBP has been shown to be a useful marker for renal function as it is totally filtered by the glomeruli and reabsorbed by proximal tubules. This has made RBP a tool to study renal function in heart or kidney transplant recipients, type 1 and 2 diabetics, and in people exposed to uranium from mining operations.  Modulating RBP4 levels may lead to new strategies in treating type 2 diabetes

When in serum, the majority of RBP bound with retinol is reversibly complexed with transthyretin. This complex transports retinol to specific receptors of various tissues in the body.  Vitamin A status is reflected by serum concentration as it is homeostatically controlled and does not fall until stores are drastically reduced.  RBP has been shown to be a useful surrogate marker for retinol because of the correlation between retinol and RBP in serum, which implies that RBP may be used to monitor vitamin A deficiency (VAD). The WHO has estimated that 250 million children have moderate to severe VAD due to lack of adequate nutrition, and the rising cost of food staples around the world further exacerbates this problem.  In addition to nutritional deficiencies, infectious stresses have been shown to depress retinol concentrations and individuals with diseases such as cystic fibrosis and HIV-1 also run the risk of VAD due to the infectious stresses that contribute to the disease.  Measurement of RBP levels has also been useful in detection and characterization of diseases including hypertension and certain cancers, among other conditions.

自1982年以来，Arbor Assays的创始人和顶尖科学家一直在研究和开发高质量的检测试剂盒。我们从小就生产FDA管制的用于人类疾病检测的体外诊断产品。1991年，当我们开始为生命科学市场生产产品时，我们意识到灵敏度，速度，便利性，质量和服务与这些产品无关。我们为您更改了。2017年1月1日，Arbor Assays通过永久员工福利信托（EBT）成为全球第一家员工拥有的生命科学公司。作为EBT，董事会确保公司为客户和员工的利益而保持独立。对于客户而言，员工拥有权意味着可以依靠公司不断进行试验，创新和提供高质量的产品。对于员工而言，所有权意味着提高工作安全性和职业满意度，而不必担心Arbor Assays将被出售给更大的开发公司。