Mouse anti Human CD117Catalogue number: 1171

Clone	NU-c-kit
Isotype	IgG1
Product Type	Single-Color Reagent
Units	100 µg
Host	Mouse
Species reactivity	Human
Application	Flow Cytometry

BackgroundCD117 reacts with 8% of myelomonocytes and 75% of CD34-positive cells.The hematopoietic stem cells are included in this fraction and therefore, this gene product is considered to have an important function for differentiation and proliferation of hematopoietic cells.CD117 also reacts with placenta, brain, a part of acute myeloblastic leukemia cells and chronic myeloblastic leukemia cells, but not with acute lymphoblastic leukemia cells.MW is 145kDa.

Synonyms: CD117

Source

Immunogen: Derived from hybridization of mouse myeloma cells and spleen cellsof a BALB/c mouse, which was immunized with transfected cells with c-kit DNA in human leukemia cells (UT-7).

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

ApplicationsPBMC: Count thenumberofpositivecells in peripheral blood.Add 10µl ofMabto50µl suspended lymphocyte solution at 2 x 10^7. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyzeor fix with 0.5%v/v ofparaformaldehyde in PBSand analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Functional Analysis: Flow Cytometry Staining

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Room Temperature

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1. Escribano L, Ocqueteau M, Almeida J, Orfao A, San Miguel JF. Leuk. Lymphoma (1998, in press). 2. Ocqueteau M, Orfao A, García-Sanz R, Almeida J, González M, San Miguel JF. Br. J. Haematol. 95: 489-93(1996). 3. Bene MC, Castoldi G, Knapp W, Ludwig WD, Matutes E, Orfao A, van’t Veer MB. Leukemia 9: 1783-86 (1995). 4. Escribano L, Orfao A, Blood (1998, in press) 5. Escribano L, Orfao A, Villarrubia J, Martín F, Madruga JI, Cuevas M, Velasco JL, Rios A, San Miguel JF.Cytometry 30: 98-102 (1997) 6. Macedo A, Orfao A, Martínez A, Vidriales MB, Valverde B, López-Berges MC, San Miguel JF. Br. J. Haematol.89: 338-41 (1995).

Protein Reference(s)

Database Name: UniProt

Accession number: P10721

Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf

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