RabbitantiHumanHistoneH2A.x(pS140)Cataloguenumber:X2752P

Isotype	IgG
ProductType	PhosphorylationSite-SpecificAntibody
Units	100µg
Host	Rabbit
Speciesreactivity	Human
Application	EnzymeImmunoassayImmunohistochemistryWesternBlotting

BackgroundVarianthistoneH2AwhichreplacesconventionalH2Ainasubsetofnucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Requiredforcheckpoint-mediatedarrestofcellcycleprogressioninresponsetolowdosesofionizingrADIationandforefficientrepairofDNAdoublestrandbreaks(DSBs)specificallywhenmodifiedbyC-terminalphosphorylation.

Synonyms:H2AFX;H2AX

Source

Immunogen:PhosphopeptidederivedfromthehumanhistoneH2A.xprotein.

Product

ProductForm:AffinityPurified

Formulation:Providedassolutioninphosphatebufferedsalinewith0.08%sodiumazide

PurificationMethod:AntigenImmunoaffiinityPurification

Concentration:Seevialforconcentration

ApplicationsAntibodycanbeusedforWesternblotting(1-5µg/ml)andimmunohistochemistry(2-5µg/mlatpH6.2).Optimalconcentrationshouldbeevaluatedbyserialdilutions.

FunctionalAnalysis:WesternBlotting

PositiveControl:Humanbladder

StorageProductshouldbestoredat-20ºC.Aliquottoavoidfreeze/thawcycles

ProductStability:Productsarestableforoneyearfrompurchasewhenstoredproperly

ShippingConditions:Shipatambienttemperature,freezeuponarrival

CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubio​acceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.

References1.Paull,T.T.,etal.‘AcriticalroleforhistoneH2AXinrecruitmentofrepairfactorstonuclearfociafterDNAdamage’Curr.Biol.,10,886-895(2000)2.Kobayashi,J.,etal.‘NBS1localizestogamma-H2AXfocithroughinteractionwiththeFHA/BRCTdomain.’Curr.Biol.,12,1846-1851(2002)3.Stewart,G.S.,etal.‘MDC1isamediatorofthemammalianDNAdamagecheckpoint.’Nature,421,961-966(2003)4.Lukas,C.,etal.‘Mdc1couplesDNAdouble-strandbreakrecognitionbyNbs1withitsH2AX-dependentchromatinretention.’EMBOJ.,23,2674-2683(2004)

ProteinReference(s)

DatabaseName:SwissProt

Accessionnumber:P16104

SpeciesAccession:Human

SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf

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