siRNA to inhibit ABCC5 expression using RNA interference.

This product is provided as three 5 nmol vials (15 nmol) or 2x three 5 nmol vials (30 nmol) of lyophilized siRNA oligo duplexes. Each vial contains slightly different sequences to ensure full knockout of the gene. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. The siRNA oligos are also chemically modified (2"-OMe) for increased stability and enhanced knockdown in vitro and in vivo.

Target	ABCC5
Reactivity	Human
Tested Applications	RNAi
Host	Synthetic
Form	Lyophilized
Purity	> 97%
Quality Control	Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Storage	Shipped at 4 °C. Store at -20 °C for up to one year.
UniProt Primary AC	O15440 (UniProt, ExPASy)
Gene Symbol	ABCC5
GeneID	10057
Specificity	ABCC5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Directions for use	1. Transfect with 100 nM siRNA 48 to 72 hours prior to cell lysis.2. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube.3. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. Each vial is suitable for 250 transfections in a 24 well plate (20 pmol for each well).
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on ABCC5 siRNA RNA Interference Products RNA干扰（RNA interference, RNAi）是指在进化过程中高度保守的、由双链RNA（double-stranded RNA，dsRNA）诱发的、同源mRNA高效特异性降解的现象。基因沉默，主要有转录前水平的基因沉默(TGS)和转录后水平的基因沉默(PTGS)两类:TGS是指由于DNA修饰或染色体异染色质化等原因使基因不能正常转录;PTGS是启动了细胞质内靶mRNA序列特异性的降解机制。有时转基因会同时导致TGS和PTGS。 由于使用RNAi技术可以特异性剔除或关闭特定基因的表达，（长度超过三十的dsRNA会引起干扰素毒性）所以该技术已被广泛用于探索基因功能和传染性疾病及恶性肿瘤的治疗领域。