10-245 kDa Protein Marker contains 12 prestained proteins ranging from 10 to 245 kDa. The 25 kDa protein is covalently coupled to a green dye and the 75 kDa protein is covalently coupled to a red dye. The other 10 proteins are covalently coupled to a blue dye. Coloured protein bands can be seen in SDS-PAGE after transferring to a PDVF, nylon or nitrocellulose membrane.

Target	10-245 kDa Protein Marker
Tested Applications	SDS-PAGE, WB
Storage	Store at 4 °C for up to 3 months. For long-term storage, store at -20 °C. Avoid repeated freeze/thaw cycles.
Buffer	20 mM Tris-phosphate, pH 7.5, 2% SDS, 0.2 mM DTT, 3.6 M Urea, and 15% Glycerol.
Directions for use	3 µl/well for clear visualisation during electrophoresis on a 15-well mini-gel5 µl/well for clear visualisation during electrophoresis on a 10-well mini-gel2-3 µl/well for general Western transferring Increase the volume for thicker (> 1.5 mm) or larger gels. Do not heat, dilute or add reducing agents before loading.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on 10-245 kDa Protein Marker RNA Interference Products RNA干扰（RNA interference, RNAi）是指在进化过程中高度保守的、由双链RNA（double-stranded RNA，dsRNA）诱发的、同源mRNA高效特异性降解的现象。基因沉默，主要有转录前水平的基因沉默(TGS)和转录后水平的基因沉默(PTGS)两类:TGS是指由于DNA修饰或染色体异染色质化等原因使基因不能正常转录;PTGS是启动了细胞质内靶mRNA序列特异性的降解机制。有时转基因会同时导致TGS和PTGS。 由于使用RNAi技术可以特异性剔除或关闭特定基因的表达，（长度超过三十的dsRNA会引起干扰素毒性）所以该技术已被广泛用于探索基因功能和传染性疾病及恶性肿瘤的治疗领域。