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蚂蚁淘在线 / 品牌中心 / Exalpha / Exalpha/Goat anti Rat IgG2c (subclass specific), conjugated with FITC/GARa-IgG2c-FITC/1 ml
商品描述
Product Code
Product Name
Goat anti Rat IgG2c (subclass specific), conjugated with FITC
Size
1 ml
Price (USD)
$453
Background
The reactivity of the antiserum is directed to the subclass IgG2c as tested against rat sera and a panel of purified homogenous immunoglobulins. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Further tested on the level of sensitivity of direct and indirect immunofluorescence staining methods applied to lymphoid cells. When used on vital peripheral blood cells this immunoconjugate does not react with Fc receptors present on cell surface membranes. To identify and measure IgG2c, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:20 and 1:80.
Host
Goat
Species Reactivity
Rat
Product Usage
Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Rat IgG2c, subclass specific.
Product Type
Secondary Antibodies
Applications
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
Fields of Interest
Veterinary
Product Source
Pools of highly purified homogenous IgG2c isolated from rat serum. Freund
Product Formulation
Fluorochrome-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Product Specificity
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this product has not been tested in detail.
Storage Conditions
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4


