Mouse anti Human Caspase-9Catalogue number: X1177M

Clone	LAAP6 96 2-22
Isotype	IgG
Product Type	Monoclonal Antibody
Units	100 µg
Host	Mouse
Species reactivity	Human
Application	Western BlotWestern Blotting

BackgroundCaspases are key effectors of programmed cell death.They are synthesized as inactive proenzymes which are activated by cleavage at a specific aspartate residue to form two subunits.These subunits are normally linked together by a linker which may be involved in the regulation of the different caspases.Caspase-9 is a member of the CED-3 family and bear high similarity to caspase-3.Procaspase-9 can be activated by either caspase-3 or granzyme B, although they cleave the proenzyme to different size subunits.Cleavage by granzyme B produces an active enzyme which is capable of cleaving PARP.Also, the ability of caspase-3 to activate caspase-9 seems to suggest that caspase-9 is further downstream of caspase-3 and may be involved in later changes in cells observed undergoing apoptosis.

Synonyms: Cysteine-requiring Aspartate Protease-9; iCE-like apoptotic protease 6;Apoptotic protease Mch-6; Apoptotic protease-activating factor 3

Source

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human capase-9 protein and mouse myeloma cells.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

ApplicationsDetects proenzyme form of human Caspase-9 by Western blot analysis as well as one of the cleavage products after treatment with thapsigargin.Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: MCF-7 cell lines

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References1. Cohen, G.M., et al. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326, 1-162. Stennicke, H.R., et al. Caspase-9 can be activated without proteolytic processing. J. Biol. Chem. 1999, 274, 8359-83623. Kuida, K. Caspase-9. Int. J. Biochem. Cell Biol. 2000, 32, 121-124

Protein Reference(s)

Database Name: SwissProt

Accession number: P55211

Species Accession: Human

Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf

Exalpha公司简介Exalpha有限公司于2000年在马萨诸塞州雪莉（美国）成立。该公司主要通过开发和生产抗体来支持生命科学领域的研究人员和科学家。Exalpha公司产品领域Exalpha公司其领域涉及信号通路、凋亡、增殖、炎症、免疫学、传染病学和细胞间结构研究等方面。Exalpha主要产品线包括Proliferation&apoptosis, Enzymes&Proteins, Clinical IHC Markers , GPCRs等多个产品线，可提供给全球的科研实验室，制药企业，生物技术和诊断公司，医院等，目前旗下的代理公司遍布全球。Exalpha公司热销产品产品名称货号规格BrdU Cell Proliferation Assay KitX1327K1200Testschx 10 (Visual system homeobox 2) (NT) Polyclonal AntibodyX1180P250ugEBNA 3C Polyclonal AntibodyF125P250ugSURF1 Polyclonal AntibodyX1562P100ugBnip3L (IN) (nix) (bcl 2 family) Polyclonal AntibodyX1120P100ugchx 10 (Visual system homeobox 2) (CT) Polyclonal AntibodyX1179P250ugMicrophthalmia Transcription Factor (MiTF) Monoclonal AntibodyX1405M100ugEBNA 3A Polyclonal AntibodyF115P250ugCD5 PE Single-Color Reagent0054100TestsPTP mu Monoclonal AntibodyP100M100ugCD117 FITC Single-Color Reagent1172100TestsKv1.5 Potassium Channel Polyclonal AntibodyX1501P100ugN-SMase Activation Associated Factor Antigen Immunoaffinity Purified PolyclonalX2354P100ugChicken anti Glutathione S Transferase (GST), conjugated with HRPAGST-HRP0.1 mgCD7 PE Single-Color Reagent0074100TestsProtein Phosphatase 1 alpha Polyclonal AntibodyP105P100ugCD2 PE Single-Color Reagent0024100TestsKv1.6Potassium Channel Polyclonal AntibodyX1502P100ugDegenerative Spermatocyte Homolog 2 (DEGS2) Antigen Immunoaffinity Purified PolyclonalX2356P100ugCD8 PE Single-Color Reagent0084100TestsProtein Phosphatase 1 beta Polyclonal AntibodyP110P100ug